Holda J H, Maier T, Claman H N
Department of Medicine, University of Colorado School of Medicine, Denver 80262.
Transplantation. 1988 Apr;45(4):772-7. doi: 10.1097/00007890-198804000-00021.
Natural suppressor (NS) cells are capable of suppressing immunological responses in a nonspecific manner. Previously, we have described NS cells in the spleens of mice undergoing chronic graft-versus-host disease (GVHD) and also in normal B10.D2 bone marrow (BM). NS cells obtained from these environments appear dependent upon lymphokines for their ability to manifest suppression. In this report, with anti-IFN-gamma antibody, we show that IFN-gamma is necessary for NS cell activation. Anti-IFN-gamma antibody is able to remove the ability of NS cells to suppress a concanavalin A (Con A) proliferation assay. Also, anti-IFN-gamma antibody removes the ability of rIL-2, lectin-free Con A supernate (CAS), and recombinant IFN-gamma (rIFN-gamma) to enhance NS suppression of lipopolysaccharide response. By these criteria, IFN-gamma is required for NS cell activation, and rIL-2 may act indirectly by its ability to stimulate IFN-gamma synthesis. These results are discussed in the context of the immuno-suppression seen in human BM transplantation.
天然抑制细胞（NS细胞）能够以非特异性方式抑制免疫反应。此前，我们曾描述过慢性移植物抗宿主病（GVHD）小鼠脾脏以及正常B10.D2骨髓（BM）中的NS细胞。从这些环境中获得的NS细胞表现出的抑制能力似乎依赖于淋巴因子。在本报告中，我们使用抗γ干扰素抗体表明，γ干扰素是NS细胞激活所必需的。抗γ干扰素抗体能够消除NS细胞抑制刀豆蛋白A（Con A）增殖试验的能力。此外，抗γ干扰素抗体还能消除重组白细胞介素-2（rIL-2）、无凝集素Con A上清液（CAS）和重组γ干扰素（rIFN-γ）增强NS细胞对脂多糖反应抑制的能力。根据这些标准，γ干扰素是NS细胞激活所必需的，而rIL-2可能通过刺激γ干扰素合成的能力间接发挥作用。我们将在人类骨髓移植中所见免疫抑制的背景下讨论这些结果。
