Chen Wanqun, Yu Yaya, Yang Naikun, Zhu Jingli, Li Ke, Li Ruocun, Su Wenqiao, Luo Lina, Hu Ling, Chen Gengxin, Deng Haixia
Department of Gastroenterology, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510120, P.R. China.
Discipline of Integrated Chinese and Western Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China.
Oncol Lett. 2018 Mar;15(3):3621-3629. doi: 10.3892/ol.2018.7757. Epub 2018 Jan 9.
Gastric cancer (GC) is one of the most common types of cancer and a leading cause of cancer-associated mortality. MicroRNAs (miRNAs/miRs) are demonstrated to function as oncomiRs or tumor-suppressor-miRs in GC. miR-7 has been identified to be a tumor suppressor of GC by targeting epidermal growth factor receptor (EGFR). In our previous study, Yangzheng Sanjie Decoction (YZSJD), a traditional Chinese formula, was identified to be effective in alleviating the symptoms and even postponing turnover of precancerous lesions. To elucidate the mechanism of YZSJD, the present study evaluated the effects of YZSJD of the GC MKN-45 cell line and investigated the underlying molecular mechanisms using YZSJD-containing serum (YCS). The expression of miR-7 in GC, normal and adjacent tissue samples was examined. The results demonstrated that YCS inhibited proliferation by inducing cell cycle arrest at the S phase, and significantly induced apoptosis compared with the control group. miR-7 was significantly downregulated in GC tissues compared with the matched ones. Using reverse transcription-quantitative polymerase chain reaction and western blot analysis, the expression of miR-7 was inversely associated with EGFR. This indicates that YCS inhibits proliferation and induces apoptosis of GC cells mediated by miR-7 targeting EGFR, which may be one of the mechanisms whereby YZSJD exerts its effects on GC.
胃癌（GC）是最常见的癌症类型之一，也是癌症相关死亡的主要原因。微小RNA（miRNA/miR）在胃癌中被证明可作为癌基因miR或肿瘤抑制miR发挥作用。已确定miR-7通过靶向表皮生长因子受体（EGFR）成为胃癌的肿瘤抑制因子。在我们之前的研究中，中药方剂养正散结汤（YZSJD）被证明可有效缓解症状，甚至延缓癌前病变的转变。为阐明YZSJD的作用机制，本研究评估了YZSJD对胃癌MKN-45细胞系的影响，并使用含YZSJD的血清（YCS）研究其潜在分子机制。检测了miR-7在胃癌、正常及相邻组织样本中的表达。结果表明，YCS通过诱导细胞周期停滞于S期抑制增殖，与对照组相比显著诱导细胞凋亡。与配对组织相比，miR-7在胃癌组织中显著下调。通过逆转录定量聚合酶链反应和蛋白质印迹分析，miR-7的表达与EGFR呈负相关。这表明YCS通过miR-7靶向EGFR抑制胃癌细胞增殖并诱导其凋亡，这可能是YZSJD对胃癌发挥作用的机制之一。
