Hai-Xia Deng, Ya-Ya Yu, Ao-Qiang Zhou, Jing-Li Zhu, Li-Na Luo, Geng-Xin Chen, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong Province, China.
World J Gastroenterol. 2017 Aug 14;23(30):5538-5548. doi: 10.3748/wjg.v23.i30.5538.
To explore the let-7a-mediated anti-cancer effect of Yangzheng Sanjie decoction (YZSJD) in gastric cancer (GC) cells.
YZSJD-containing serum (YCS) was prepared using traditional Chinese medicine serum pharmacology methods. After YCS treatment, cell proliferation and apoptosis were assessed by cell counting kit-8 assay and flow cytometry, respectively, and miRNA expression profiles were determined using qPCR arrays. Let-7a expression was examined by hybridization in GC tissues and by qPCR in GC cells. c-Myc protein expression was detected by immunohistochemistry in GC tissues, and by Western blot in cell lines.
YZSJD significantly inhibited proliferation and induced apoptosis in AGS and HS-746T GC cells. After treatment with YCS, the miRNA expression profiles were altered and the reduced let-7a levels in both cell lines were up-regulated, accompanied by a decrease in c-Myc expression. Moreover, decreased let-7a expression and increased c-Myc expression were observed during the progression of gastric mucosa cancerization.
YZSJD inhibits proliferation and induces apoptosis of GC cells by restoring the aberrant expression of let-7a and c-Myc.
探讨养正散结方（YZSJD）通过 let-7a 抑制胃癌（GC）细胞的抗癌作用。
采用中药血清药理学方法制备含 YZSJD 的血清（YCS）。用细胞计数试剂盒-8 法和流式细胞术分别检测 YCS 处理后的细胞增殖和凋亡情况，并用 qPCR 阵列检测 miRNA 表达谱。通过杂交检测 GC 组织中的 let-7a 表达，并用 qPCR 检测 GC 细胞中的 let-7a 表达。用免疫组织化学法检测 GC 组织中的 c-Myc 蛋白表达，用 Western blot 检测细胞系中的 c-Myc 蛋白表达。
YZSJD 显著抑制 AGS 和 HS-746T GC 细胞的增殖并诱导其凋亡。用 YCS 处理后，miRNA 表达谱发生改变，两种细胞系中的 let-7a 水平降低得到上调，同时 c-Myc 表达下降。此外，在胃癌黏膜癌变过程中观察到 let-7a 表达降低和 c-Myc 表达增加。
YZSJD 通过恢复 let-7a 和 c-Myc 的异常表达抑制 GC 细胞的增殖并诱导其凋亡。
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