Sakane Ayuko, Yoshizawa Shin, Yokota Hideo, Sasaki Takuya
Department of Biochemistry, Tokushima University Graduate School of Medical Sciences, Tokushima, Japan.
Image Processing Research Team, RIKEN Center for Advanced Photonicsm RIKEN, Wako, Japan.
Front Cell Dev Biol. 2018 Feb 5;6:4. doi: 10.3389/fcell.2018.00004. eCollection 2018.
Collective cell migration is observed during morphogenesis, angiogenesis, and wound healing, and this type of cell migration also contributes to efficient metastasis in some kinds of cancers. Because collectively migrating cells are much better organized than a random assemblage of individual cells, there seems to be a kind of order in migrating clusters. Extensive research has identified a large number of molecules involved in collective cell migration, and these factors have been analyzed using dramatic advances in imaging technology. To date, however, it remains unclear how myriad cells are integrated as a single unit. Recently, we observed unbalanced collective cell migrations that can be likened to either precision dancing or , Japanese traditional dancing similar to the style at Rio Carnival, caused by the impairment of the conformational change of JRAB/MICAL-L2. This review begins with a brief history of image-based computational analyses on cell migration, explains why quantitative analysis of the stylization of collective cell behavior is difficult, and finally introduces our recent work on JRAB/MICAL-L2 as a successful example of the multidisciplinary approach combining cell biology, live imaging, and computational biology. In combination, these methods have enabled quantitative evaluations of the "dancing style" of collective cell migration.
在形态发生、血管生成和伤口愈合过程中可观察到集体细胞迁移，并且这种类型的细胞迁移在某些癌症的高效转移中也发挥作用。由于集体迁移的细胞比单个细胞的随机组合组织得更好，因此在迁移簇中似乎存在一种秩序。广泛的研究已经确定了大量参与集体细胞迁移的分子，并且利用成像技术的巨大进步对这些因素进行了分析。然而，迄今为止，尚不清楚无数细胞是如何作为一个单一单元整合在一起的。最近，我们观察到不平衡的集体细胞迁移，其类似于精准舞蹈，或者类似于里约热内卢狂欢节风格的日本传统舞蹈，这是由JRAB/MICAL-L2构象变化受损引起的。本综述首先简要介绍基于图像的细胞迁移计算分析的历史，解释为什么对集体细胞行为的风格化进行定量分析很困难，最后介绍我们最近关于JRAB/MICAL-L2的工作，作为结合细胞生物学、实时成像和计算生物学的多学科方法的成功范例。综合起来，这些方法能够对集体细胞迁移的“舞蹈风格”进行定量评估。
