Lopez Mary Susan, Vemuganti Raghu
Department of Neurosurgery, University of Wisconsin-Madison, Madison, WI, USA.
Methods Mol Biol. 2018;1717:101-113. doi: 10.1007/978-1-4939-7526-6_9.
The middle cerebral artery occlusion (MCAO) model is widely used for inducing a focal cerebral ischemic insult (stroke) in rodents. Here, we describe a method for transient MCAO technique without craniotomy in both mice and rats. In our laboratory, this technique yields consistent secondary brain damage that evolves over a period of 3-7 days of reperfusion after transient MCAO. We also describe the methods for analyzing postischemic motor dysfunction and infarct volume in rodents subjected to transient MCAO.
大脑中动脉闭塞（MCAO）模型被广泛用于在啮齿动物中诱导局灶性脑缺血损伤（中风）。在此，我们描述一种在小鼠和大鼠中无需开颅的短暂性MCAO技术方法。在我们实验室，该技术会产生持续的继发性脑损伤，这种损伤在短暂性MCAO后再灌注的3至7天内逐渐发展。我们还描述了分析接受短暂性MCAO的啮齿动物缺血后运动功能障碍和梗死体积的方法。
