Department of Biology, Faculty of Science, Urmia University, Urmia, Iran.
Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Urmia University of Medical Science, Nazloo Street, P.O. Box 57147-83734, Urmia, Iran.
Biol Trace Elem Res. 2018 Oct;185(2):424-432. doi: 10.1007/s12011-018-1270-9. Epub 2018 Feb 21.
Colorectal cancer is one of the most common causes of mortality in the world while malnutrition is responsible for one third of the problem. Selenium has been recommended for prevention of colorectal cancer. The present study was conducted to investigate the effect of selenium-enriched Saccharomyces cerevisiae in reducing colorectal cancer progression in rats. Five groups of 170-200-g weight rats (n = 40) including healthy and cancer controls, Saccharomyces cerevisiae, selenium, and selenium-enriched Saccharomyces cerevisiae-treated groups were examined. All animals except healthy control group received 40 mg 1,2-dimethylhydrazine (DMH) per kilogram weight of rat twice a week. The healthy group received normal saline, and synchronously, selenium group received soluble selenium (4 mg/mL), Saccharomyces cerevisiae and selenium-enriched groups received yeast with the density of 5 × 10 CFU/mL by daily gavage. All treatments were carried out for 5 weeks after the last injection. Animals were autopsied, and aberrant crypt foci (ACF) of ejected colon were studied in the 40th week. Microscopic sections were prepared for hematoxylin and eosin. Furthermore, immunohistochemical staining of CD31, BCL2, and P53 antibodies was performed. Macroscopic and microscopic evaluations showed that DMH had the least destructive effect in selenium-enriched Saccharomyces cerevisiae group compared to other groups. Selenium-enriched Saccharomyces cerevisiae reduces colorectal cancer progression by various mechanisms such as reduction in the number and size of ACF and alteration in the function of the proteins such as P53, BCL2, and CD31.
结直肠癌是世界上最常见的死亡原因之一，而营养不良是造成这一问题的三分之一原因。硒已被推荐用于预防结直肠癌。本研究旨在探讨富硒酿酒酵母对减少大鼠结直肠肿瘤进展的作用。共检查了五组 170-200 克体重的大鼠（n=40），包括健康对照组和肿瘤对照组、酿酒酵母组、硒组和富硒酿酒酵母组。除健康对照组外，所有动物均接受每周两次、每公斤体重 40mg1,2-二甲基肼（DMH）的处理。健康对照组给予生理盐水，同时硒组给予可溶硒（4mg/mL），酿酒酵母组和富硒酿酒酵母组通过每日灌胃给予密度为 5×10 CFU/mL 的酵母。所有治疗均在最后一次注射后进行 5 周。动物进行尸检，并研究第 40 周排出的结肠的异常隐窝病灶（ACF）。制备苏木精和伊红切片。此外，还进行了 CD31、BCL2 和 P53 抗体的免疫组织化学染色。宏观和微观评估表明，与其他组相比，富硒酿酒酵母组中 DMH 的破坏性最小。富硒酿酒酵母通过减少 ACF 的数量和大小以及改变 P53、BCL2 和 CD31 等蛋白质的功能等多种机制来降低结直肠癌的进展。
