Feizabadi Farideh, Minaiyan Mohsen, Taheri Azade
Novel Drug Delivery Systems Research Center, Department of Pharmaceutics, Faculty of Pharmacy, Isfahan University of Medical sciences, Isfahan, Iran.
Department of Pharmacology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Curr Drug Deliv. 2018;15(6):840-849. doi: 10.2174/1567201815666180220100154.
Nanofibers such as bacterial cellulose nanofibers (BC-NFs) have gained increasing attention for use in wound dressings. Topical application of arginine can stimulate wound healing significantly.
In order to promote the wound healing process, arginine functionalized BC-NFs containing gel (Arg-BC-NFs gel) was prepared by the electrostatic attachment of arginine on the surface of BCNFs.
The effect of pH was evaluated on the amount of the attached arginine on the BC-NFs surface. The attachment of arginine on BC-NFs surface was investigated by FTIR spectroscopy. The morphology of Arg-BC-NFs was evaluated using FESEM. The viscosity and spreadability of Arg-BC-NFs and the release of arginine from Arg-BC-NFs were evaluated. The effectiveness of Arg-BC-NFs gel was assessed in a full thickness wound model in rats. Re-epithelization, collagen deposition and neovascularization were investigated in the wound tissues using histological and immunohistochemical analysis.
FTIR spectra and the zeta potential of BC-NFs confirmed the surface modification of BC-NFs by arginine. FESEM images showed the nanofibrous structure of Arg-BC-NFs. The release of arginine from Arg-BC-NFs gel was in a sustained release manner for 24 h. The appropriate viscosity and spreadability of Arg-BC-NFs gel confirmed its easy topical application. In vivo studies revealed that Arg-BCNFs gel promoted wound closure at a faster rate than BC-NFs gel and arginine solution. Moreover, faster and more organized re-epithelialization, angiogenesis and collagen deposition were achieved in Arg-BC-NFs gel treated group in comparison to other groups.
Arg-BC-NFs gel can be introduced as an effective wound dressing for acute wounds.
诸如细菌纤维素纳米纤维（BC-NFs）之类的纳米纤维在伤口敷料中的应用越来越受到关注。局部应用精氨酸可显著促进伤口愈合。
为了促进伤口愈合过程，通过精氨酸在BC-NFs表面的静电附着制备了含精氨酸功能化BC-NFs的凝胶（Arg-BC-NFs凝胶）。
评估了pH对BC-NFs表面附着精氨酸量的影响。通过傅里叶变换红外光谱（FTIR）研究精氨酸在BC-NFs表面的附着情况。使用场发射扫描电子显微镜（FESEM）评估Arg-BC-NFs的形态。评估了Arg-BC-NFs的粘度和铺展性以及精氨酸从Arg-BC-NFs中的释放情况。在大鼠全层伤口模型中评估了Arg-BC-NFs凝胶的有效性。使用组织学和免疫组织化学分析研究伤口组织中的再上皮化、胶原蛋白沉积和新血管形成。
BC-NFs的FTIR光谱和zeta电位证实了精氨酸对BC-NFs的表面修饰。FESEM图像显示了Arg-BC-NFs的纳米纤维结构。精氨酸从Arg-BC-NFs凝胶中的释放呈持续释放24小时的方式。Arg-BC-NFs凝胶具有适当的粘度和铺展性，证实了其易于局部应用。体内研究表明，Arg-BC-NFs凝胶促进伤口闭合的速度比BC-NFs凝胶和精氨酸溶液更快。此外，与其他组相比，Arg-BC-NFs凝胶治疗组实现了更快且更有序的再上皮化、血管生成和胶原蛋白沉积。
Arg-BC-NFs凝胶可作为急性伤口的有效伤口敷料引入。
