College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, P R China.
Analyst. 2018 Mar 12;143(6):1462-1467. doi: 10.1039/c8an00048d.
This work presents a novel rapid and sensitive label-free electrochemical method for the detection of bacteria on surface nanostructures. A simple electrochemical deposition and calcination method is employed to prepare different gold nanostructures on FTO substrate. The sensor based on nanostructure gold exhibits excellent linear relation between E. coli DH5α bacteria and the changes of ΔR, especially FTO-GEDC-D30, with a correction coefficient R = 0.998. Both the spectrophotometric (OD methods) and fluorescence-staining methods also verified the reliability of electrochemical impedance spectroscopy (EIS) methods for evaluating the antibacterial activity of the gold nanostructure.
本工作提出了一种新颖的快速、灵敏的无标记电化学方法，用于检测表面纳米结构上的细菌。采用简单的电化学沉积和煅烧方法，在 FTO 基底上制备不同的金纳米结构。基于纳米结构金的传感器在 E. coli DH5α 细菌和 ΔR 变化之间表现出优异的线性关系，特别是 FTO-GEDC-D30，校正系数 R = 0.998。分光光度法（OD 法）和荧光染色法也验证了电化学阻抗谱（EIS）方法评估金纳米结构抗菌活性的可靠性。
