Shill Daniel D, Lansford Kasey A, Hempel Hannah K, Call Jarrod A, Murrow Jonathan R, Jenkins Nathan T
Department of Kinesiology, University of Georgia, Athens, GA, USA.
Regenerative Bioscience Center, University of Georgia, Athens, GA, USA.
Exp Physiol. 2018 May 1;103(5):693-700. doi: 10.1113/EP086644. Epub 2018 Mar 26.
What is the central question of this study? What is the effect of exercise intensity on circulating microparticle populations in young, healthy men and women? What is the main finding and its importance? Acute, moderate-intensity continuous exercise and high-intensity interval exercise altered distinct microparticle populations during and after exercise in addition to a sex-specific response in CD62E microparticles. The microparticles studied contribute to cardiovascular disease progression, regulate vascular function and facilitate new blood vessel formation. Thus, characterizing the impact of intensity on exercise-induced microparticle responses advances our understanding of potential mechanisms underlying the beneficial vascular adaptations to exercise.
Circulating microparticles (MPs) are biological vectors of information within the cardiovascular system that elicit both deleterious and beneficial effects on the vasculature. Acute exercise has been shown to alter MP concentrations, probably through a shear stress-dependent mechanism, but evidence is limited. Therefore, we investigated the effect of exercise intensity on plasma levels of CD34 and CD62E MPs in young, healthy men and women. Blood samples were collected before, during and after two energy-matched bouts of acute treadmill exercise: interval exercise (10 × 1 min intervals at ∼95% of maximal oxygen uptake V̇O2max) and continuous exercise (65% V̇O2max). Continuous exercise, but not interval exercise, reduced CD62E MP concentrations in men and women by 18% immediately after exercise (from 914.5 ± 589.6 to 754.4 ± 390.5 MPs μl ; P < 0.05), suggesting that mechanisms underlying exercise-induced CD62E MP dynamics are intensity dependent. Furthermore, continuous exercise reduced CD62E MPs in women by 19% (from 1030.6 ± 688.1 to 829.9 ± 435.4 MPs μl ; P < 0.05), but not in men. Although interval exercise did not alter CD62E MPs per se, the concentrations after interval exercise were higher than those observed after continuous exercise (P < 0.05). Conversely, CD34 MPs did not fluctuate in response to short-duration acute continuous or interval exercise in men or women. Our results suggest that exercise-induced MP alterations are intensity dependent and sex specific and impact MP populations differentially.
本研究的核心问题是什么？运动强度对年轻健康男性和女性循环微颗粒群体有何影响？主要发现及其重要性是什么？急性中等强度持续运动和高强度间歇运动在运动期间及运动后改变了不同的微颗粒群体，此外，在CD62E微颗粒方面存在性别特异性反应。所研究的微颗粒会促进心血管疾病进展、调节血管功能并促进新血管形成。因此，明确强度对运动诱导的微颗粒反应的影响，有助于我们进一步了解运动对血管产生有益适应性变化的潜在机制。
循环微颗粒（MPs）是心血管系统内的生物信息载体，对脉管系统既有有害影响也有有益影响。急性运动已被证明会改变MPs浓度，可能是通过剪切应力依赖性机制，但相关证据有限。因此，我们研究了运动强度对年轻健康男性和女性血浆中CD34和CD62E MPs水平的影响。在两次能量匹配的急性跑步机运动（间歇运动：10个1分钟间歇，强度约为最大摄氧量V̇O2max的95%；持续运动：65%V̇O2max）的前、中、后采集血样。持续运动而非间歇运动使男性和女性运动后即刻的CD62E MPs浓度降低了18%（从914.5±589.6降至754.4±390.5 MPs/μl；P<0.05），这表明运动诱导的CD62E MPs动态变化机制与强度有关。此外，持续运动使女性的CD62E MPs降低了19%（从1030.6±688.1降至829.9±435.4 MPs/μl；P<0.05），但对男性无此影响。虽然间歇运动本身并未改变CD62E MPs，但间歇运动后的浓度高于持续运动后的浓度（P<0.05）。相反，男性或女性的CD34 MPs在短时间急性持续运动或间歇运动后并未波动。我们的结果表明，运动诱导的MPs改变与强度有关且具有性别特异性，对MPs群体的影响存在差异。
