[Investigation of two blood parasitic protozoa infection in farmed in Guangxi Zhuang Autonomous Region].

OBJECTIVE

To investigate the infection situation of blood parasitic protozoa in farmed in an animal breeding ground in Nanning, Guangxi Zhuang Autonomous Region, so as to provide the evidence for the prevention and control of human blood parasitic protozoa.

METHODS

A total of 993 blood samples from farmed were collected and stored on FTA cards. Among them, 550 thin blood smears were made. Each 10 samples were mixed in groups, and then the spp and spp. in the blood of were detected by Nest-PCR and PCR, respectively. The positive groups were tested individually. The thin blood smears stained with Giemsa were examined microscopically when PCR reported the samples were positive.

RESULTS

When detected by Nest-PCR, the positive rate of was 6.95% (69/993); only 1 positive sample with was detected by PCR. Among the 22 positive thin blood smears detected by PCR, 16 were determined with by microscopic examinations, on which the ring forms could be observed in the erythrocytes, but no hemozoin.

CONCLUSIONS

The positive rate of in in Guangxi Zhuang Autonomous Region is high, and the animal may play a role as a reservoir host in the transmission of . In the screening of with low infection density, Nest-PCR has a higher sensitivity.