[Preparation of recombinant retrovirus pRevTRE-E77.43 and its protective effect in a mouse model of infection].
作者信息
Fan-Sheng Zeng, Sai-Qun Luo, De-Hui Xiong, Yuan-Jing Yu, Ying-Jun Qian, Zhi-Qiang Qin
机构信息
Key Laboratory of Control Technique for Schistosoma and Pathogen Infection for Dongting Lake Region, Yiyang Medical College, Yiyang 413002, China.
Molecular Biology Research Center, College of Life Science, Central South University, China.
出版信息
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2017 Dec 27;29(6):730-735. doi: 10.16250/j.32.1374.2017164.
OBJECTIVE
To explore the biological functions of E77.43, a gene segment of , in treating infection.
METHODS
Recombinant retroviral vectors of pRevTREE77.43 was constructed, and recombinant retroviral vectors were transfected into PA317 cells, and the stable cell lines were obtained by hygromycin screening, followed by the packaging, concentration and purification of recombinant retrovirus. The virus was transferred to the mice infected by via intravenous or intraperitoneal injection, through which the express of target gene and the treatment function in vivo were observed.
RESULTS
The experiment showed the recombinant virus injected mice could efficiently express E77.43 on the 7th day after the injection which lasted for fortyfive days thereafter. A significant reduction in adult worms (31.0%) and a high reduction (35.0%) in liver eggs were induced by pRevTREE77.43, while the reduction in adult worms and that in liver eggs was 1.2% and 0.9% induced by pRevTRE respectively ( = 3.524, 9.485, both ＜0.01).
CONCLUSIONS
pRevTREE77.43 could be used for the treatment of infection, indicating that E77.43 may involve in the natural resistance of to infection.
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