Zun-Fu Wang, Zhi-Hua Jiang, Bing-Xue Yu, Dong-Sheng Zhou, Yuan Lin, Wen-Qian Tang
Fourth People's Hospital of Nanning, Guangxi Zhuang Autonomous Region, Nanning 530023, China.
Guangxi Center for Disease Control and Prevention, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2016 May 31;28(5):550-553. doi: 10.16250/j.32.1374.2016038.
To understand the infection status and gene types of among HIV/AIDS patients in Guangxi Zhuang Autonomous Region.
The fecal samples were collected from 285 HIV/AIDS cases in Nanning, Guilin, Qinzhou, Baise, Hechi cities of Guangxi and 150 HIV negative persons in Nanning City. The modified acid-fast staining and nested-PCR based on 18S rRNA were employed to detect the infection status of . The nested PCR products were sequenced, and the homology searches and identification for the gene types of were done by DNAStar software.
The infection rate of in HIV/AIDS patients was 0.70% (2/285), and the rate of those with chronic diarrhea was 6.67% (2/30), the latter was significantly higher than that of the HIV negative persons (0, 0/150) ( = 0.002). Both the two HIV/AIDS patients infected with were from Guilin City. By molecular identification, the strains which the above 2 patients were infected with were and respectively.
co-infection can be found in HIV/AIDS patients in Guangxi. The genotypes of the infection strains include and
了解广西壮族自治区艾滋病病毒/艾滋病（HIV/AIDS）患者中[某种微生物名称未给出]的感染状况及基因类型。
采集广西南宁、桂林、钦州、百色、河池市285例HIV/AIDS患者的粪便样本以及南宁市150例HIV阴性者的粪便样本。采用改良抗酸染色法及基于18S rRNA的巢式聚合酶链反应（PCR）检测[某种微生物名称未给出]的感染状况。对巢式PCR产物进行测序，并通过DNAStar软件对[某种微生物名称未给出]的基因类型进行同源性搜索和鉴定。
HIV/AIDS患者中[某种微生物名称未给出]的感染率为0.70%（2/285），慢性腹泻患者中的感染率为6.67%（2/30），后者显著高于HIV阴性者（0，0/150）（P = 0.002）。两名感染[某种微生物名称未给出]的HIV/AIDS患者均来自桂林市。经分子鉴定，上述2例患者感染的[某种微生物名称未给出]菌株分别为[具体菌株名称1]和[具体菌株名称2]。
广西HIV/AIDS患者中可发现[某种微生物名称未给出]合并感染。感染菌株的基因型包括[具体基因型1]和[具体基因型2]
