Medical Microbiology & Molecular Biology Lab., Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
Future Microbiol. 2018 Mar;13:437-454. doi: 10.2217/fmb-2017-0207. Epub 2018 Feb 22.
The objective of the study was to look the efficacy of fractionated light against Streptococcus mutans biofilm.
MATERIALS & METHODS: Antibiofilm assays (crystal violet, congo red), electron microscopic, confocal and spectroscopic studies were performed to check the effect of fractionated light.
6-6.5 log reduction of planktonic and 3.6-4.2 log reduction in biofilm were observed after irradiation with fractionated as compared with continuous light. Increased permeability to propidium iodide and leakage of cellular constituent validate the greater antibiofilm effect of fractionated light. Spectroscopic studies confirmed the relative contribution of type I and type II photochemistry.
Phenothiazinium dyes have a potential against bacterial biofilm in combination with light fractionation and it offers new opportunities to explore its clinical implication.
本研究旨在观察分段光对变形链球菌生物膜的疗效。
采用结晶紫、刚果红抗生物膜检测法、电子显微镜、共聚焦显微镜和光谱学研究来观察分段光的作用。
与连续光照射相比，分段光照射后浮游生物的减少量为 6-6.5 对数，生物膜减少量为 3.6-4.2 对数。碘化丙啶通透性增加和细胞成分泄漏证实了分段光更强的抗生物膜作用。光谱学研究证实了 I 型和 II 型光化学的相对贡献。
吩噻嗪染料与光分段结合具有抗细菌生物膜的潜力，为探索其临床意义提供了新的机会。
