Centre for Protein Science, Design and Engineering, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Manauli, Mohali, Punjab, India.
IUBMB Life. 2018 Apr;70(4):260-266. doi: 10.1002/iub.1725. Epub 2018 Feb 22.
Vibrio cholerae cytolysin (VCC) is a membrane-damaging protein toxin with potent cytolytic/cytotoxic activity against wide range of eukaryotic cells. VCC is a β-barrel pore-forming toxin (β-PFT), and it inflicts damage to the target cell membranes by forming transmembrane heptameric β-barrel pores. To exert pore-forming activity, VCC must bind to the cell membranes in an efficient manner. Efficient interaction with the cell membranes is an essential pre-requisite to trigger subsequent structural/conformational and organizational changes in the toxin molecules leading toward formation of the transmembrane oligomeric β-barrel pores. Based on the large numbers of studies investigating the mode of action of VCC, it is now evident that VCC is capable of using multiple distinct mechanisms to recognize and bind to the membrane components and cell surface molecules. In this review article, we present an overview of our current understanding regarding the membrane interaction mechanisms of VCC, and their functional implications for the pore-forming activity of the toxin. © 2018 IUBMB Life, 70(4):260-266, 2018.
霍乱弧菌细胞溶素（VCC）是一种具有广泛真核细胞细胞毒性/细胞毒性活性的膜损伤蛋白毒素。VCC 是一种 β-桶孔形成毒素（β-PFT），通过形成跨膜七聚体 β-桶状孔对靶细胞膜造成损伤。为了发挥形成孔的活性，VCC 必须以有效的方式与细胞膜结合。与细胞膜的有效相互作用是触发毒素分子随后的结构/构象和组织变化的必要前提，导致形成跨膜寡聚 β-桶状孔。基于大量研究霍乱弧菌细胞溶素作用模式的研究，现在很明显，霍乱弧菌细胞溶素能够使用多种不同的机制来识别和结合膜成分和细胞表面分子。在这篇综述文章中，我们概述了我们目前对霍乱弧菌细胞溶素的膜相互作用机制的理解，以及它们对毒素形成孔的活性的功能意义。
