Bagheri-Khoulenjani Shadab, Mirzadeh Hamid
Department of Polymer Engineering, Amirkabir University of Technology, 424 Hafez-Ave., P.O. Box 15875-4413, Tehran, Iran.
Prog Biomater. 2012 Nov 14;1(1):4. doi: 10.1186/2194-0517-1-4.
Biomaterial surface modification is an efficient method to improve and control blood component-material interactions. In the present study, two different methods (ArF excimer laser irradiation and radio-frequency (RF) plasma treatment) were applied in separate procedures to create a vast range of physicochemical characteristics on the surface of polystyrene (PS) and investigate their effects on blood compatibility of treated surfaces. Atomic force microscopy (AFM) and Fourier transmission infrared analysis were applied to study the morphology and chemical characteristics of treated samples in comparison with those of the untreated PS. Contact angle and surface tension measurements with two different solvents were used to evaluate the wettability and surface energy of the treated PS films. The effect of the physicochemical properties of the PS surface on blood compatibility was investigated using lactate dehydrogenase (LDH) method. AFM studies showed that after laser treatment, some distinctive nanostructures are created on the surface of PS. The data from contact angle measurements demonstrated that ArF excimer laser irradiation and RF plasma treatment created surfaces with a vast range of properties in the wettability point of view. The LDH results revealed that after surface modification by laser irradiation and plasma treatment, blood compatibility of PS films was enhanced. In addition, these results offered that the most blood compatible samples were those which irradiated with 5 pulses of laser and the one treated 4 minutes in oxygen plasma.
生物材料表面改性是改善和控制血液成分与材料相互作用的有效方法。在本研究中，分别采用两种不同方法（ArF准分子激光辐照和射频（RF）等离子体处理）对聚苯乙烯（PS）表面进行处理，以赋予其广泛的物理化学特性，并研究这些特性对处理后表面血液相容性的影响。与未处理的PS相比，采用原子力显微镜（AFM）和傅里叶变换红外分析研究处理后样品的形态和化学特性。使用两种不同溶剂进行接触角和表面张力测量，以评估处理后PS薄膜的润湿性和表面能。采用乳酸脱氢酶（LDH）法研究PS表面物理化学性质对血液相容性的影响。AFM研究表明，激光处理后，PS表面形成了一些独特的纳米结构。接触角测量数据表明，从润湿性角度来看，ArF准分子激光辐照和RF等离子体处理创造了具有广泛性质的表面。LDH结果显示，经激光辐照和等离子体处理进行表面改性后，PS薄膜的血液相容性得到增强。此外，这些结果表明，血液相容性最佳的样品是那些经5次激光脉冲辐照的样品以及在氧等离子体中处理4分钟的样品。
