Mahmoodi Mahboobeh, Hashemi Peyman Mahmoodi, Imani Rana
Department of Materials and Mechanic, Yazd Branch, Islamic Azad University, Yazd, Iran.
Department of Biomedical Engineering, College of Engineering and Technical, Yazd Science and Research Branch, Islamic Azad University, Yazd, Iran.
Prog Biomater. 2014 Jun 14;3(1):25. doi: 10.1007/s40204-014-0025-8.
For the purposes of this study, hydroxyapatite (HA)-AlO-TiO nanobiomaterial with significant surface properties and biocompatibility capable of forming surface apatite was fabricated by cold-press and sintering method. Samples were examined for hardness and porosity. The results showed that in terms of hardness and porosity, sample A (50 wt% TiO-30 wt% HA-20 wt% AlO) was superior to sample B (30 wt% TiO-50 wt% HA-20 wt% AlO), and also the density of nanobiomaterial was close to natural bone density. Bioactivity of the samples in a simulated body fluid (SBF) was investigated. Then, after immersing the samples in SBF solution for a period of 7 days, sample B exhibited greater ability to form calcium phosphate compounds on the surface as compared to sample A. In addition, in vitro studies showed that MG-67 osteoblast-like cells attached and spread on the samples surface. The results showed that cells proliferated in greater numbers on the sample B as compared to the sample A. Finally, X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray analysis were performed to identify phases, study microstructure, and determine percentage of elements, respectively. The results revealed that considering their different properties, both nanobiomaterials can be used in medical applications.
为了本研究的目的，通过冷压和烧结法制备了具有显著表面性质和生物相容性且能够形成表面磷灰石的羟基磷灰石（HA）-AlO-TiO纳米生物材料。对样品进行了硬度和孔隙率检测。结果表明，在硬度和孔隙率方面，样品A（50 wt% TiO-30 wt% HA-20 wt% AlO）优于样品B（30 wt% TiO-50 wt% HA-20 wt% AlO），并且纳米生物材料的密度接近天然骨密度。研究了样品在模拟体液（SBF）中的生物活性。然后，将样品在SBF溶液中浸泡7天后，与样品A相比，样品B在表面形成磷酸钙化合物的能力更强。此外，体外研究表明，MG-67成骨样细胞附着并铺展在样品表面。结果表明，与样品A相比，样品B上细胞增殖数量更多。最后，分别进行了X射线衍射、扫描电子显微镜和能量色散X射线分析，以鉴定相、研究微观结构和确定元素百分比。结果表明，考虑到它们的不同性质，两种纳米生物材料均可用于医学应用。
