Ebrahimian-Hosseinabadi Mehdi, Etemadifar Mohammadreza, Ashrafizadeh Fakhredin
Department of Biomedical Engineering, Faculty of Engineering, University of Isfahan, Isfahan, Iran.
Orthopedic Department, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
J Med Signals Sens. 2016 Oct-Dec;6(4):237-242.
In this paper, preparation, bioactivity, and osteoblast cell behavior of cortical bone derived nano-biphasic calcium phosphate (nano-BCP) are presented. The calcined bovine bone samples with the addition of di-ammonium hydrogen phosphate were heated at 700°C for 100 min, and thus nano-BCP with the composition of 63/37 hydroxyapatite (HA)/β-tricalcium phosphate (β-TCP) was produced. Scanning electron microscopy (SEM) images, energy dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD) analysis of immersed samples in simulated body fluid (SBF) solution showed that a uniform layer was formed on the surface after 7 days with the chemical composition of HA. The results indicated that the nano-BCP sample developed excellent bioactivity after 28 days. The nano-BCP samples showed better cell proliferation compared to pure HA samples. After 7 days in cell culture, the prepared nano-BCP (HA/β-TCP) exhibited the maximum proliferation of the MG-63 osteoblast cells.
本文介绍了皮质骨衍生纳米双相磷酸钙（nano-BCP）的制备、生物活性和成骨细胞行为。添加磷酸氢二铵的煅烧牛骨样品在700°C下加热100分钟，从而制备出组成为63/37羟基磷灰石（HA）/β-磷酸三钙（β-TCP）的纳米BCP。对浸泡在模拟体液（SBF）溶液中的样品进行扫描电子显微镜（SEM）图像、能量色散X射线光谱（EDS）和X射线衍射（XRD）分析表明，7天后表面形成了一层均匀的HA化学成分层。结果表明，纳米BCP样品在28天后具有优异的生物活性。与纯HA样品相比，纳米BCP样品显示出更好的细胞增殖。细胞培养7天后，制备的纳米BCP（HA/β-TCP）表现出MG-63成骨细胞的最大增殖。
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