Jetty Devarathnam, Sharma Ashok Kumar, Kumar Naveen, Shrivastava Sameer, Sonal B, Rai R B
Division of Surgery, Indian Veterinary Research Institute, Izatnagar, 243122, Uttar Pradesh, India.
Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, 243122, Uttar Pradesh, India.
Prog Biomater. 2014 Dec;3(2-4):115-122. doi: 10.1007/s40204-014-0027-6. Epub 2014 Sep 23.
In the present study, biocompatibility of native, acellular, 1,4-butanediol diglycidylether and 1-ethyl-3-(3-dimethyl aminopropyl carbodiimide (EDC) cross-linked acellular aortic grafts was evaluated following subcutaneous implantation in guinea pigs. Biocompatibility was evaluated based on macroscopic, histopathological observations and immune responses elicited by the implanted grafts. Results showed that macroscopically, no abnormal cellular reaction was observed at the host-graft junction in any of the implanted animals. Histopathological observations revealed that the inflammatory response was mild during first 15 days post-implantation and increased at 30 days post-implantation in acellular and cross-linked tissues. By day 60, marked ingrowth of host tissue was observed in EDC cross-linked acellular aortic grafts. ELISA and lymphocyte proliferation assay revealed that animals implanted with EDC grafts showed least immune response when compared to others. Therefore, it was concluded that EDC cross-linked acellular aortic grafts were more compatible and had better handling qualities than the other cross-linked grafts.
在本研究中，将天然、去细胞、1,4 - 丁二醇二缩水甘油醚和1 - 乙基 - 3 -（3 - 二甲基氨基丙基）碳二亚胺（EDC）交联的去细胞主动脉移植物在豚鼠皮下植入后，评估其生物相容性。基于宏观、组织病理学观察以及植入移植物引发的免疫反应来评估生物相容性。结果显示，从宏观上看，在任何植入动物的宿主 - 移植物交界处均未观察到异常细胞反应。组织病理学观察表明，去细胞和交联组织在植入后的前15天炎症反应较轻，在植入后30天炎症反应增强。到第60天时，在EDC交联的去细胞主动脉移植物中观察到宿主组织明显向内生长。酶联免疫吸附测定（ELISA）和淋巴细胞增殖试验表明，与其他移植物相比，植入EDC移植物的动物表现出最小的免疫反应。因此，得出结论，EDC交联的去细胞主动脉移植物比其他交联移植物具有更高的相容性和更好的操作性能。
