Kumar Vineet, Kumar Naveen, Gangwar Anil K, Singh Himani, Singh Rajendra
Division of Surgery, Indian Veterinary Research Institute, Uttar Pradesh, India.
Division of Surgery, Indian Veterinary Research Institute, Uttar Pradesh, India.
J Surg Res. 2015 Aug;197(2):436-46. doi: 10.1016/j.jss.2015.04.080. Epub 2015 Apr 29.
Collagen-rich extracellular matrix from land-based mammalian tissues is increasingly used in regenerative medicine. However, its uses are associated with risk of disease transfer and may carry an ethnocultural stigma. In the present study, collagen-rich acellular swim bladder matrix (ASBM) from Rohu fish was prepared using sodium deoxycholate and crosslinked with 1,4-butanediol diglycidyl ether (BDDGE). Wound healing potential of ASBM and ASBM-BDDGE was compared in full-thickness skin wounds in rabbits.
Four full-thickness skin wounds (20 × 20 mm(2) each) were created on the dorsum of 18 rabbits and randomly divided into three equal groups. Wounds were left open, repaired with ASBM and ASBM-BDDGE in groups sham (I), ASBM (II), and ASBM-BDDGE (III), respectively. Planimetry, contracture, immunologic, and histologic observations were carried out to evaluate wound healing.
Significantly (P < 0.05) lesser wound contraction was observed in ASBM (II) and ASBM-BDDGE (III) groups compared with sham (I) group. Total immunoglobulin G response in rabbit sera was decreased significantly (P < 0.05) in the ASBM-BDDGE (III) group compared with ASBM (II) group by enzyme-linked immunosorbent assay. Stimulation index of peripheral blood lymphocytes was decreased significantly (P < 0.05) in the ASBM-BDDGE (III) group compared with ASBM (II) group by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Histologically, improved epithelialization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in ASBM (II) and ASBM-BDDGE (III) groups as early as on postimplantation day 21.
Findings of this study indicate that BDDGE crosslinked ASBM derived from Rohu fish has potential for the clinical applications. Furthermore, it is expected that their clinical applications will not be limited by ethnocultural stigma.
来自陆生哺乳动物组织的富含胶原蛋白的细胞外基质在再生医学中的应用越来越广泛。然而，其应用与疾病传播风险相关，并且可能带有种族文化污名。在本研究中，使用脱氧胆酸钠制备了来自印度鲤鱼的富含胶原蛋白的无细胞鱼鳔基质（ASBM），并与1,4 - 丁二醇二缩水甘油醚（BDDGE）交联。在兔的全层皮肤伤口中比较了ASBM和ASBM - BDDGE的伤口愈合潜力。
在18只兔的背部制造四个全层皮肤伤口（每个20×20 mm²），并随机分为三个相等的组。伤口敞开，分别在假手术组（I）、ASBM组（II）和ASBM - BDDGE组（III）中用ASBM和ASBM - BDDGE修复。进行平面测量、挛缩、免疫学和组织学观察以评估伤口愈合。
与假手术组（I）相比，ASBM组（II）和ASBM - BDDGE组（III）观察到明显（P < 0.05）较小的伤口收缩。通过酶联免疫吸附测定法，与ASBM组（II）相比，ASBM - BDDGE组（III）中兔血清中的总免疫球蛋白G反应显著降低（P < 0.05）。通过3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐测定法，与ASBM组（II）相比，ASBM - BDDGE组（III）中外周血淋巴细胞的刺激指数显著降低（P < 0.05）。组织学上，早在植入后第21天，在ASBM组（II）和ASBM - BDDGE组（III）中观察到上皮化、新血管形成、纤维组织增生改善以及胶原纤维排列最佳。
本研究结果表明，BDDGE交联的来自印度鲤鱼的ASBM具有临床应用潜力。此外，预计它们的临床应用不会受到种族文化污名的限制。
