Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
Biochem Biophys Res Commun. 2018 Mar 4;497(2):790-796. doi: 10.1016/j.bbrc.2018.02.157. Epub 2018 Feb 19.
Circular RNAs are newly identified RNAs that regulate gene expression and execute various biological activities. Facet joint osteoarthritis (FJOA) is a severe facet joint cartilage degeneration disease that induces chronic low back pain to a larger population. The molecular mechanisms of FJOA, especially the presence and changes of circular RNAs in FJOA have not been investigated. In the current study, we analyzed RNA deep sequencing outcomes from healthy control group and FJOA group, identified and annotated circular RNAs, and determined the expressions of circular RNAs. Differentially expressed circular RNAs in the FJOA group were then screened and the host genes of these differentially expressed circular RNAs were functional analyzed by Gene Ontology (GO), Kyoto Enrichment of Genes and Genomes (KEGG), and protein-protein interaction network analysis. Seven circRNAs were arbitrarily selected to confirm the trustworthiness of these profiles by quantitative qRT-PCR. The potential binding sites of microRNAs (miRNAs) and identified circular RNAs were also predicted. Our current study provided valuable bioinformation regarding circular RNAs in FJOA and expanded our knowledge of FJOA.
环状 RNA 是新鉴定的 RNA，可调节基因表达并执行各种生物活性。小关节骨关节炎 (FJOA) 是一种严重的小关节软骨退化疾病，导致更大人群的慢性下腰痛。FJOA 的分子机制，特别是 FJOA 中环状 RNA 的存在和变化，尚未得到研究。在本研究中，我们分析了来自健康对照组和 FJOA 组的 RNA 深度测序结果，鉴定和注释了环状 RNA，并确定了环状 RNA 的表达。然后筛选 FJOA 组中差异表达的环状 RNA，并通过基因本体论 (GO)、京都基因与基因组百科全书 (KEGG) 和蛋白质-蛋白质相互作用网络分析对这些差异表达的环状 RNA 的宿主基因进行功能分析。通过定量 qRT-PCR 随机选择了 7 个 circRNAs 来验证这些图谱的可信度。还预测了鉴定出的环状 RNA 和 microRNAs (miRNAs) 的潜在结合位点。我们目前的研究为 FJOA 中的环状 RNA 提供了有价值的生物信息，并扩展了我们对 FJOA 的认识。
