Department of Rheumatology, 1st Affiliated Hospital of China Medical University, Shen Yang, China.
Department of Rheumatology, Dazhou Central Hospital, Da Zhou, China.
Sci Rep. 2018 Feb 22;8(1):3462. doi: 10.1038/s41598-018-21859-z.
We measured the interleukin-34 (IL-34) level in sera from patients with systemic lupus erythematosus (SLE) and discoid lupus erythematosus (DLE) using an enzyme-linked immunosorbent assay (ELISA). Blood tests, including assays to determine C-reactive protein (CRP), complement (C) 3, C4, immunoglobulin (Ig) A, IgG, IgM, anti-double-stranded DNA antibody (Anti-dsDNA Ab) and hemoglobin (Hb) levels and white blood cell (WBC) and platelet (PLT) counts, were performed using standard methods. Lupus nephritis (LN) was diagnosed according to the American College of Rheumatology (ACR) renal criteria. The SLE disease activity was scored using the SLE Disease Activity Index (SLEDAI). Among the 110 SLE cases, IL-34 could be detected in 79 cases (71.8%). IL-34 was barely detected in the control group. The serum level of IL-34 was significantly higher in the SLE group. No change was observed in the serum IL-34 concentration in the SLE patients regardless of LN status. Correlations were observed between the serum IL-34 level and the disease activity parameters. The SLE patients with detectable IL-34 levels had higher SLEDAI and IgG concentrations and lower C3 and Hb levels than patients with undetectable IL-34 levels. Therefore, IL-34 could be a potential disease activity marker for SLE.
我们使用酶联免疫吸附测定（ELISA）检测了系统性红斑狼疮（SLE）和盘状红斑狼疮（DLE）患者血清中的白细胞介素-34（IL-34）水平。血液测试包括测定 C 反应蛋白（CRP）、补体（C）3、C4、免疫球蛋白（Ig）A、IgG、IgM、抗双链 DNA 抗体（Anti-dsDNA Ab）和血红蛋白（Hb）水平以及白细胞（WBC）和血小板（PLT）计数，均采用标准方法进行。狼疮肾炎（LN）根据美国风湿病学会（ACR）肾脏标准进行诊断。使用 SLE 疾病活动指数（SLEDAI）对 SLE 疾病活动进行评分。在 110 例 SLE 病例中，可检测到 79 例（71.8%）的 IL-34。对照组几乎未检测到 IL-34。SLE 组的血清 IL-34 水平明显升高。无论 LN 状态如何，SLE 患者的血清 IL-34 浓度均无变化。血清 IL-34 水平与疾病活动参数之间存在相关性。可检测到 IL-34 水平的 SLE 患者的 SLEDAI 和 IgG 浓度较高，而 C3 和 Hb 水平较低。因此，IL-34 可能是 SLE 的潜在疾病活动标志物。
