• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

激酶依赖性前馈回路影响 CREBB 的稳定性和长期记忆形成。

A kinase-dependent feedforward loop affects CREBB stability and long term memory formation.

机构信息

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.

Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Bethesda, United States.

出版信息

Elife. 2018 Feb 23;7:e33007. doi: 10.7554/eLife.33007.

DOI:10.7554/eLife.33007
PMID:29473541
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5825208/
Abstract

In , long-term memory (LTM) requires the cAMP-dependent transcription factor CREBB, expressed in the mushroom bodies (MB) and phosphorylated by PKA. To identify other kinases required for memory formation, we integrated Trojan exons encoding into genes encoding putative kinases and selected for genes expressed in MB. These lines were screened for learning/memory deficits using UAS-RNAi knockdown based on an olfactory aversive conditioning assay. We identified a novel, conserved kinase, (, , in human), the loss of which severely affects 3 hr memory and 24 hr LTM, but not learning. Remarkably, memory is lost upon removal of the MP protein in adult MB but restored upon its reintroduction. Overexpression of MP in MB significantly increases LTM in wild-type flies showing that is a limiting factor for LTM. We show that PKA phosphorylates MP and that both proteins synergize in a feedforward loop to control CREBB levels and LTM. key words: Drosophila, Mushroom bodies, SBK1, deGradFP, T2A-GAL4, MiMIC.

摘要

在果蝇中，长时记忆（LTM）需要 cAMP 依赖性转录因子 CREBB，其在蘑菇体（MB）中表达并被 PKA 磷酸化。为了鉴定其他参与记忆形成的激酶，我们将编码 Trojan 外显子的序列整合到编码假定激酶的基因中，并选择在 MB 中表达的基因。这些品系通过基于嗅觉厌恶条件反射测定的 UAS-RNAi 敲低筛选学习/记忆缺陷。我们鉴定了一种新型的保守激酶， （,, 在人类），其缺失严重影响 3 小时记忆和 24 小时 LTM，但不影响学习。值得注意的是，在成年 MB 中去除 MP 蛋白会导致记忆丧失，但重新引入后会恢复。在野生型果蝇的 MB 中过表达 MP 会显著增加 LTM，表明 是 LTM 的限制因素。我们表明 PKA 磷酸化 MP，并且这两种蛋白质在正反馈回路中协同作用以控制 CREBB 水平和 LTM。关键词：果蝇，蘑菇体，SBK1，deGradFP，T2A-GAL4，MiMIC。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/643d84fd5573/elife-33007-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/e1fa770e9416/elife-33007-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/c0f95d8209dd/elife-33007-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/ec1aed969c01/elife-33007-fig1-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/697e612e1aa9/elife-33007-fig1-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/aaf35501c818/elife-33007-fig1-figsupp4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/67a593bfbcad/elife-33007-fig1-figsupp5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/a36786e191d3/elife-33007-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/49425db4efff/elife-33007-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/a35f73b16fd2/elife-33007-fig2-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/a55f9327a423/elife-33007-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/6a8320079155/elife-33007-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/816f47ee42ce/elife-33007-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/8a1a7d64bb7a/elife-33007-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/59b4f6301864/elife-33007-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/db46ba3cc740/elife-33007-fig5-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/643d84fd5573/elife-33007-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/e1fa770e9416/elife-33007-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/c0f95d8209dd/elife-33007-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/ec1aed969c01/elife-33007-fig1-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/697e612e1aa9/elife-33007-fig1-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/aaf35501c818/elife-33007-fig1-figsupp4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/67a593bfbcad/elife-33007-fig1-figsupp5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/a36786e191d3/elife-33007-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/49425db4efff/elife-33007-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/a35f73b16fd2/elife-33007-fig2-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/a55f9327a423/elife-33007-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/6a8320079155/elife-33007-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/816f47ee42ce/elife-33007-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/8a1a7d64bb7a/elife-33007-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/59b4f6301864/elife-33007-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/db46ba3cc740/elife-33007-fig5-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7328/5825208/643d84fd5573/elife-33007-fig6.jpg

相似文献

1
A kinase-dependent feedforward loop affects CREBB stability and long term memory formation.激酶依赖性前馈回路影响 CREBB 的稳定性和长期记忆形成。
Elife. 2018 Feb 23;7:e33007. doi: 10.7554/eLife.33007.
2
CREBB repression of protein synthesis in mushroom body gates long-term memory formation in .CREBB 对蘑菇体中蛋白质合成的抑制作用控制着长期记忆的形成。
Proc Natl Acad Sci U S A. 2022 Dec 13;119(50):e2211308119. doi: 10.1073/pnas.2211308119. Epub 2022 Dec 5.
3
Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit.多个神经元在蘑菇体回路中编码依赖 CrebB 的奖赏性长期记忆。
Elife. 2018 Oct 22;7:e39196. doi: 10.7554/eLife.39196.
4
CREBA and CREBB in two identified neurons gate long-term memory formation in .CREBA 和 CREBB 在两种确定的神经元中控制着长期记忆的形成。
Proc Natl Acad Sci U S A. 2021 Sep 14;118(37). doi: 10.1073/pnas.2100624118.
5
Spatio-temporal in vivo recording of dCREB2 dynamics in Drosophila long-term memory processing.果蝇长期记忆处理过程中dCREB2动态变化的时空体内记录。
Neurobiol Learn Mem. 2015 Feb;118:80-8. doi: 10.1016/j.nlm.2014.11.010. Epub 2014 Nov 22.
6
Visualizing long-term memory formation in two neurons of the Drosophila brain.可视化果蝇大脑中两个神经元的长期记忆形成。
Science. 2012 Feb 10;335(6069):678-85. doi: 10.1126/science.1212735.
7
Environmental Light Is Required for Maintenance of Long-Term Memory in .环境光是维持. 长期记忆所必需的。
J Neurosci. 2020 Feb 12;40(7):1427-1439. doi: 10.1523/JNEUROSCI.1282-19.2019. Epub 2020 Jan 13.
8
Drosophila ORB protein in two mushroom body output neurons is necessary for long-term memory formation.果蝇 ORB 蛋白在两个蘑菇体输出神经元中对于长期记忆的形成是必需的。
Proc Natl Acad Sci U S A. 2013 May 7;110(19):7898-903. doi: 10.1073/pnas.1216336110. Epub 2013 Apr 22.
9
Middle-Term Memory: Amnesiac is Required for PKA Activation in the Mushroom Bodies, a Function Modulated by Neprilysin 1.中期记忆：PKA 在蘑菇体中的激活需要健忘症，这一功能由 Neprilysin 1 调节。
J Neurosci. 2020 May 20;40(21):4219-4229. doi: 10.1523/JNEUROSCI.2311-19.2020. Epub 2020 Apr 17.
10
Fasting launches CRTC to facilitate long-term memory formation in Drosophila.禁食可激活 CRTC 促进果蝇的长期记忆形成。
Science. 2013 Jan 25;339(6118):443-6. doi: 10.1126/science.1227170.

引用本文的文献

1
Feeding a rich diet supplemented with the translation inhibitor cycloheximide decreases lifespan and ovary size in Drosophila.用富含翻译抑制剂环己亚胺的饮食喂养会缩短果蝇的寿命并减小其卵巢大小。
Biol Open. 2024 Nov 15;13(11). doi: 10.1242/bio.061697. Epub 2024 Nov 25.
2
Feeding a rich diet supplemented with the translation inhibitor cycloheximide decreases lifespan and ovary size in Drosophila.喂食富含翻译抑制剂环己酰亚胺的食物会缩短果蝇的寿命并减小其卵巢大小。
bioRxiv. 2024 Aug 19:2024.08.19.608713. doi: 10.1101/2024.08.19.608713.
3
Use-Dependent, Untapped Dual Kinase Signaling Localized in Brain Learning Circuitry.

本文引用的文献

1
Importin-7 mediates memory consolidation through regulation of nuclear translocation of training-activated MAPK in Drosophila.输入蛋白-7通过调节果蝇中训练激活的丝裂原活化蛋白激酶的核转位来介导记忆巩固。
Proc Natl Acad Sci U S A. 2016 Mar 15;113(11):3072-7. doi: 10.1073/pnas.1520401113. Epub 2016 Feb 29.
2
A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila.MiMIC文库可用于在果蝇中对基因进行标记以及对蛋白质进行可逆的、空间和时间上的敲低。
Elife. 2015 Mar 31;4:e05338. doi: 10.7554/eLife.05338.
3
Plug-and-play genetic access to drosophila cell types using exchangeable exon cassettes.
脑学习回路中存在的、具有功能依赖性的、未被开发的双重激酶信号。
J Neurosci. 2024 Mar 20;44(12):e1126232024. doi: 10.1523/JNEUROSCI.1126-23.2024.
4
An optimized temporally controlled Gal4 system in reveals degeneration caused by adult-onset neuronal Vps13D knockdown.一种优化的时间控制Gal4系统揭示了成年期神经元Vps13D基因敲低所导致的退化。
Front Neurosci. 2023 Jun 29;17:1204068. doi: 10.3389/fnins.2023.1204068. eCollection 2023.
5
Learning and memory using Drosophila melanogaster: a focus on advances made in the fifth decade of research.利用黑腹果蝇进行学习和记忆研究：聚焦于研究的第五十个年头所取得的进展。
Genetics. 2023 Aug 9;224(4). doi: 10.1093/genetics/iyad085.
6
CREBB repression of protein synthesis in mushroom body gates long-term memory formation in .CREBB 对蘑菇体中蛋白质合成的抑制作用控制着长期记忆的形成。
Proc Natl Acad Sci U S A. 2022 Dec 13;119(50):e2211308119. doi: 10.1073/pnas.2211308119. Epub 2022 Dec 5.
7
Functional Dissection of Protein Kinases in Sexual Development and Female Receptivity of .蛋白质激酶在性发育和雌性接受性中的功能剖析 。 需注意，你提供的原文不完整，句末的“.”后面似乎缺少具体内容。
Front Cell Dev Biol. 2022 Jun 9;10:923171. doi: 10.3389/fcell.2022.923171. eCollection 2022.
8
Circadian programming of the ellipsoid body sleep homeostat in .椭圆囊体睡眠稳态的昼夜节律编程。
Elife. 2022 Jun 23;11:e74327. doi: 10.7554/eLife.74327.
9
Temporally and Spatially Localized PKA Activity within Learning and Memory Circuitry Regulated by Network Feedback.网络反馈调节的学习记忆回路中具有时空间局域化的 PKA 活性。
eNeuro. 2022 Apr 1;9(2). doi: 10.1523/ENEURO.0450-21.2022. Print 2022 Mar-Apr.
10
Loss of IRF2BPL impairs neuronal maintenance through excess Wnt signaling.IRF2BPL的缺失通过过量的Wnt信号传导损害神经元维持。
Sci Adv. 2022 Jan 21;8(3):eabl5613. doi: 10.1126/sciadv.abl5613. Epub 2022 Jan 19.
使用可交换外显子盒对果蝇细胞类型进行即插即用的基因访问。
Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
4
Functional neuroanatomy of Drosophila olfactory memory formation.果蝇嗅觉记忆形成的功能神经解剖学
Learn Mem. 2014 Sep 15;21(10):519-26. doi: 10.1101/lm.034363.114. Print 2014 Oct.
5
Time of day influences memory formation and dCREB2 proteins in Drosophila.昼夜时间影响果蝇的记忆形成和 dCREB2 蛋白。
Front Syst Neurosci. 2014 Mar 31;8:43. doi: 10.3389/fnsys.2014.00043. eCollection 2014.
6
Nuclear gating of a Drosophila dCREB2 activator is involved in memory formation.果蝇 dCREB2 激活物的核门控参与记忆形成。
Neurobiol Learn Mem. 2013 Nov;106:258-67. doi: 10.1016/j.nlm.2013.09.006. Epub 2013 Sep 25.
7
CASK regulates CaMKII autophosphorylation in neuronal growth, calcium signaling, and learning.CASK 调节神经元生长、钙信号和学习中的 CaMKII 自身磷酸化。
Front Mol Neurosci. 2013 Sep 11;6:27. doi: 10.3389/fnmol.2013.00027. eCollection 2013.
8
dCREB2-mediated enhancement of memory formation.dCREB2 介导的记忆形成增强。
J Neurosci. 2013 Apr 24;33(17):7475-87. doi: 10.1523/JNEUROSCI.4387-12.2013.
9
In vivo circadian oscillation of dCREB2 and NF-κB activity in the Drosophila nervous system.果蝇神经系统中 dCREB2 和 NF-κB 活性的体内昼夜节律振荡。
PLoS One. 2012;7(10):e45130. doi: 10.1371/journal.pone.0045130. Epub 2012 Oct 15.
10
A permissive role of mushroom body α/β core neurons in long-term memory consolidation in Drosophila.蘑菇体α/β 核神经元在果蝇长时记忆巩固中的许可作用。
Curr Biol. 2012 Nov 6;22(21):1981-9. doi: 10.1016/j.cub.2012.08.048. Epub 2012 Oct 11.