Rezadehbashi Maryam, Baldwin Susan A
Chemical and Biological Engineering, University of British Columbia, 2360 East Mall, Vancouver, BC V6T 1Z3, Canada.
Microorganisms. 2018 Feb 23;6(1):16. doi: 10.3390/microorganisms6010016.
Biochemical reactors (BCRs) based on the stimulation of sulphate-reducing microorganisms (SRM) are emerging semi-passive remediation technologies for treatment of mine-influenced water. Their successful removal of metals and sulphate has been proven at the pilot-scale, but little is known about the types of SRM that grow in these systems and whether they are diverse or restricted to particular phylogenetic or taxonomic groups. A phylogenetic study of four established pilot-scale BCRs on three different mine sites compared the diversity of SRM growing in them. The mine sites were geographically distant from each other, nevertheless the BCRs selected for similar SRM types. Clostridia SRM related to spp. known to be tolerant to high concentrations of copper were members of the core microbial community. Members of the SRM family Desulfobacteraceae were dominant, particularly those related to . Methanogens were dominant archaea and possibly were present at higher relative abundances than SRM in some BCRs. Both hydrogenotrophic and acetoclastic types were present. There were no strong negative or positive co-occurrence correlations of methanogen and SRM taxa. Knowing which SRM inhabit successfully operating BCRs allows practitioners to target these phylogenetic groups when selecting inoculum for future operations.
基于硫酸盐还原微生物（SRM）刺激的生化反应器（BCR）是用于处理受矿山影响水体的新兴半被动修复技术。它们在中试规模上成功去除金属和硫酸盐已得到证实，但对于在这些系统中生长的SRM类型以及它们是多样的还是局限于特定的系统发育或分类群知之甚少。对三个不同矿山场地的四个已建成的中试规模BCR进行的系统发育研究比较了其中生长的SRM的多样性。这些矿山场地在地理上彼此相距遥远，但所选的BCR具有相似的SRM类型。与已知耐受高浓度铜的梭菌属相关的SRM是核心微生物群落的成员。脱硫杆菌科SRM家族的成员占主导地位，特别是与[具体菌种]相关的那些。产甲烷菌是主要的古菌，在一些BCR中，其相对丰度可能高于SRM。氢营养型和乙酸裂解型都存在。产甲烷菌和SRM分类群之间没有强烈的负相关或正相关共现关系。了解哪些SRM栖息在成功运行的BCR中，使从业者在为未来操作选择接种物时能够针对这些系统发育群体。
