Food Technology Department, Faculty of Engineering, Bina Nusantara University, Jakarta, Indonesia, 11480.
Centre for Healthy Eating & Food Innovation, Maastricht University - campus Venlo, Venlo, The Netherlands.
J Appl Microbiol. 2018 Jun;124(6):1604-1609. doi: 10.1111/jam.13740. Epub 2018 Mar 23.
To test the effect of microencapsulation on the survival of two probiotic strains isolated from Dadih, Indonesian fermented buffalo milk, in a dynamic, computer-controlled in vitro model of the upper gastrointestinal (GI) tract (TIM-1), simulating human adults.
Free or microencapsulated probiotics, Lactobacillus plantarum IS-10506 or Enterococcus faecium IS-27526, resuspended in milk were studied for survival in the complete TIM-1 system (stomach + small intestine) or in the gastric compartment of TIM-1 only. Hourly samples collected after the ileal-caecal valve or after the pylorus were plated on MRS agar (for Lactobacillus) or S&B agar (for Enterococcus). Survival of the free cells after transit through the complete TIM-1 system was on average for the E. faecium and L. plantarum 15·0 and 18·5% respectively. Survival of the microencapsulated E. faecium and L. plantarum was 15·7 and 84·5% respectively. The free cells were further assessed in only the gastric compartment of TIM-1. E. faecium and L. plantarum showed an average survival of 39 and 32%, respectively, after gastric passage.
There is similar sensitivity to gastric acid as well as survival after complete upper GI tract transit of free cells, but microencapsulation only protected L. plantarum.
Survival of microencapsulated L. plantarum IS-10506 is increased compared to free cells in a validated in vitro model of the upper GI tract. It increases its use as an ingredient of functional foods.
在模拟成人胃肠道的动态、计算机控制的体外模型（TIM-1）中，检测微胶囊化对两种从印度尼西亚发酵水牛乳 Dadih 中分离的益生菌菌株在胃肠道（GI）上部生存的影响。
研究了悬浮在牛奶中的游离或微囊化益生菌（植物乳杆菌 IS-10506 或屎肠球菌 IS-27526）在完整的 TIM-1 系统（胃+小肠）或 TIM-1 的胃部分中的生存情况。在回盲瓣或幽门后每小时收集样品，在 MRS 琼脂（用于乳酸菌）或 S&B 琼脂（用于肠球菌）上平板。游离细胞通过完整的 TIM-1 系统后，屎肠球菌和植物乳杆菌的存活率平均分别为 15.0%和 18.5%。微囊化屎肠球菌和植物乳杆菌的存活率分别为 15.7%和 84.5%。游离细胞进一步在 TIM-1 的胃部分中进行评估。胃通过后，屎肠球菌和植物乳杆菌的平均存活率分别为 39%和 32%。
游离细胞对胃酸的敏感性以及在上胃肠道完全通过后的存活率相似，但微囊化仅保护了植物乳杆菌。
与游离细胞相比，在经过验证的上胃肠道体外模型中，微囊化植物乳杆菌 IS-10506 的存活率增加。这增加了其作为功能性食品成分的用途。
