Li Peiyuan, Lei Jiajun, Hu Guangsheng, Chen Xuanmin, Liu Zhifeng, Yang Jing
Department of Gastroenterology, The First Affiliated Hospital of University of South China, Hengyang, China.
Xiangya School of Medicine, Central South University, Changsha, China.
Front Physiol. 2019 Feb 8;10:28. doi: 10.3389/fphys.2019.00028. eCollection 2019.
This study mainly investigated the effect of matrine on TNBS-induced intestinal inflammation in mice. TNBS treatment caused colonic injury and gut inflammation. Matrine (1, 5, and 10 mg/kg) treatment alleviated colonic injury and gut inflammation via reducing bleeding and diarrhea and downregulating cytokines expression (IL-1β and TNF-α). Meanwhile, serum immunoglobulin G (IgG) was markedly reduced in TNBS treated mice, while 5 and 10 mg/kg matrine alleviated IgG reduction. Fecal microbiota was tested using 16S sequencing and the results showed that TNBS caused gut microbiota dysbiosis, while matrine treatment markedly improved gut microbiota communities (i.e., Bacilli and Mollicutes). Functional analysis showed that cell motility, nucleotide metabolism, and replication and repair were markedly altered in the TNBS group, while matrine treatment significantly affected cell growth and death, membrane transport, nucleotide metabolism, and replication and repair. In conclusion, matrine may serve as a protective mechanism in TNBS-induced colonic inflammation and the beneficial effect may be associated with gut microbiota.
本研究主要探讨苦参碱对三硝基苯磺酸（TNBS）诱导的小鼠肠道炎症的影响。TNBS处理导致结肠损伤和肠道炎症。苦参碱（1、5和10mg/kg）处理通过减少出血和腹泻以及下调细胞因子表达（IL-1β和TNF-α）减轻了结肠损伤和肠道炎症。同时，TNBS处理的小鼠血清免疫球蛋白G（IgG）明显降低，而5和10mg/kg苦参碱减轻了IgG的降低。使用16S测序检测粪便微生物群，结果表明TNBS导致肠道微生物群失调，而苦参碱处理显著改善了肠道微生物群落（即芽孢杆菌和柔膜菌）。功能分析表明，TNBS组细胞运动、核苷酸代谢以及复制和修复明显改变，而苦参碱处理显著影响细胞生长和死亡、膜转运、核苷酸代谢以及复制和修复。总之，苦参碱可能在TNBS诱导的结肠炎症中起到保护作用，其有益作用可能与肠道微生物群有关。
