Department of Pathology from Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
J Low Genit Tract Dis. 2018 Apr;22(2):147-151. doi: 10.1097/LGT.0000000000000367.
The aim of the study was to compare, using a proteomic approach, cervicovaginal fluid (CVF) proteins of women with bacterial vaginosis (BV) with those presenting normal microbiota.
A total of 309 reproductive-aged women were cross-sectionally enrolled. Participants were tested for vaginal candidosis, Trichomonas vaginalis, Chlamydia trachomatis, and Neisseria gonorrhoeae and excluded if positive. Vaginal microbiota was classified microscopically according to Nugent criteria in normal, intermediate, and BV. Randomly selected CVF samples of 29 women with BV and an equal number with normal microbiota were subjected to proteomic analysis. Thus, a total of 58 CVF samples were evaluated using shotgun liquid chromatography-tandem mass spectrometry in a Q-Tof PREMIER API mass spectrometer (MicroMass/Waters) for peptide detection and relative quantification.
Of the 309 women enrolled, 63 (20.4%) were excluded after testing positive for at least one of the tested co-infections or because of low-quality samples. Microscopic classification of vaginal microbiota on the remaining 246 samples revealed that 132 women (53.6%) had normal microbiota, 33 (13.4%) had intermediate microbiota, and 81 (33.0%) had BV. Proteomic analysis of CVF of 58 randomly selected women with normal microbiota (n = 29) or BV (n = 29) successfully identified 74 proteins. In addition, the comparison of abundance of those proteins between the groups showed that the following five (6.7%) were enriched in BV: neutrophil elastase, kaliocin-1, neutrophil defensin-1, Ig lambda-2 chain C regions, and protein S100-A7. All of which have a recognized role in host's immunity.
Exclusive finding of BV affects immunity-related CVF components of reproductive-aged women.
本研究旨在通过蛋白质组学方法比较细菌性阴道病（BV）患者和正常菌群患者的宫颈阴道分泌物（CVF）蛋白。
共纳入 309 名育龄期妇女进行横断面研究。对所有参与者进行阴道假丝酵母菌病、阴道毛滴虫、沙眼衣原体和淋病奈瑟菌检测，若检测结果阳性则排除在外。根据 Nugent 标准，采用显微镜检查法将阴道微生物群分类为正常、中间和 BV。随机选择 29 名 BV 患者和 29 名正常菌群患者的 CVF 样本进行蛋白质组学分析。因此，使用 shotgun 液相色谱-串联质谱法（Q-Tof PREMIER API 质谱仪，MicroMass/Waters）对总共 58 份 CVF 样本进行肽检测和相对定量。
在 309 名入组的女性中，有 63 名（20.4%）至少有一种检测到的合并感染阳性或因样本质量差而被排除在外。对剩余 246 名女性的阴道微生物群进行显微镜分类后发现，132 名（53.6%）女性的阴道微生物群正常，33 名（13.4%）女性的阴道微生物群处于中间状态，81 名（33.0%）女性患有 BV。对 58 名随机选择的具有正常菌群（n = 29）或 BV（n = 29）的女性的 CVF 进行蛋白质组学分析，成功鉴定出 74 种蛋白质。此外，对两组之间这些蛋白质丰度的比较显示，以下五种（6.7%）在 BV 中富集：中性粒细胞弹性蛋白酶、卡利ocin-1、中性粒细胞防御素-1、Ig 轻链-2 区和 S100-A7 蛋白。所有这些蛋白都在宿主免疫中具有公认的作用。
BV 的独特发现影响了育龄期女性与免疫相关的 CVF 成分。
