Kradin R L, Zhu Y, Hales C A, Bianco C, Colvin R B
Am J Pathol. 1986 Nov;125(2):349-57.
The in vivo acquisition of coagulation plasma proteins on the surface of pulmonary macrophages was studied in guinea pigs breathing 95% oxygen. Fibrin/ogen and fibronectin appeared rapidly and concurrently on the surfaces of macrophages in the bronchoalveolar lavage fluid, which was judged by immunohistologic examination and flow cytometry. Pulmonary macrophages showed a parallel increase in the expression of a surface fibronectin receptor. Hyperoxic lung injury was accompanied by deposition of an extravascular pulmonary matrix of fibronectin and fibrin/ogen and depressed plasma levels of fibronectin. Binding to clot matrix proteins may lead to aggregation and retention of macrophages at sites of acute lung injury as well as alteration of cell function.
在吸入95%氧气的豚鼠中研究了肺巨噬细胞表面凝血血浆蛋白的体内获取情况。通过免疫组织学检查和流式细胞术判断，纤维蛋白/原和纤连蛋白迅速且同时出现在支气管肺泡灌洗液中巨噬细胞的表面。肺巨噬细胞表面纤连蛋白受体的表达也相应增加。高氧肺损伤伴有血管外肺纤连蛋白和纤维蛋白/原基质的沉积以及血浆纤连蛋白水平降低。与凝块基质蛋白的结合可能导致巨噬细胞在急性肺损伤部位聚集和滞留，以及细胞功能改变。
