Department of Chemistry , University of California , Riverside , California 92521 , United States.
School of Medicine , University of California , 900 University Avenue Riverside , California 92521 , United States.
Anal Chem. 2018 Mar 20;90(6):4196-4202. doi: 10.1021/acs.analchem.8b00393. Epub 2018 Mar 7.
The large intestine (cecum and colon) is a complex biochemical factory of vital importance to human health. It plays a major role in digestion and absorption by salvaging nutrients from polysaccharides via fermentation initiated by the bacteria that comprise the gut microbiome. We hypothesize that the intestinal epithelium absorbs a limited number of luminal metabolites with bioactive potential while actively excluding those with toxic effects. To explore this concept, we combined H NMR detection with Ussing chamber measurements of absorptive transport by rat cecum. Numerous metabolites transported across the epithelium can be measured simultaneously by H NMR, a universal detector of organic compounds, alleviating the need for fluorescent or radiolabeled compounds. Our results demonstrate the utility of this approach to delineate the repertoire of fecal solutes that are selectively absorbed by the cecum and to determine their transport rates.
大肠（盲肠和结肠）是一个复杂的生化工厂，对人类健康至关重要。它通过发酵从细菌组成的肠道微生物组中回收多糖中的营养物质，在消化和吸收方面发挥着主要作用。我们假设肠道上皮细胞吸收具有生物活性的腔室代谢物的数量是有限的，同时主动排除那些具有毒性作用的代谢物。为了探索这一概念，我们结合了 H NMR 检测和大鼠盲肠吸收转运的 Ussing 室测量。通过 H NMR 可以同时测量许多穿过上皮细胞转运的代谢物，H NMR 是一种通用的有机化合物检测器，可以减轻对荧光或放射性标记化合物的需求。我们的结果证明了这种方法的实用性，可以用来描绘盲肠选择性吸收的粪便溶质谱，并确定它们的转运速率。
