School of Molecular and Cellular Biology, Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK.
School of Molecular and Cellular Biology, Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK.
N Biotechnol. 2018 Oct 25;45:28-35. doi: 10.1016/j.nbt.2018.02.008. Epub 2018 Feb 21.
Cancer is frequently characterised by dysregulation of the cellular signalling processes that govern proliferation, survival and attachment. Understanding such dysregulation continues to present a challenge given the importance of protein-protein interactions in intracellular processes. Exploring this protein-protein interactome requires novel tools capable of discriminating between highly homologous proteins, individual domains and post-translational modifications. This review examines the potential of scaffold-based binding proteins to fulfil these requirements. It also explores protein-protein interactions in the context of intracellular signalling pathways and cancer, and demonstrates the uses of scaffold proteins as functional moderators, biosensors and imaging reagents. This review also highlights the timeliness and potential to develop international consortia to develop and validate highly specific "proteome" scaffold-based binding protein reagents with the ultimate aim of developing screening tools for studying the interactome.
癌症的一个显著特征是细胞信号过程失调，这些过程调节细胞的增殖、存活和附着。鉴于蛋白质-蛋白质相互作用在细胞内过程中的重要性，理解这种失调仍然是一个挑战。探索这种蛋白质-蛋白质相互作用组需要能够区分高度同源蛋白质、单个结构域和翻译后修饰的新型工具。这篇综述检查了基于支架的结合蛋白满足这些要求的潜力。它还探讨了细胞内信号通路和癌症中的蛋白质-蛋白质相互作用，并展示了支架蛋白作为功能调节剂、生物传感器和成像试剂的用途。本综述还强调了及时开发国际联盟的必要性，以开发和验证高度特异性的“蛋白质组”基于支架的结合蛋白试剂，最终目的是开发用于研究相互作用组的筛选工具。
