Liu Gang, Li Shubin, Yuan Haihan, Hao Mengrui, Yun Zhizhong, Zhao Jie, Ma Yuzhen, Dai Yanfeng
State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, College of Biological Sciences, Inner Mongolia University, 235 West Univ. Road, Hohhot, Inner Mongolia, 010021, China.
Inner Mongolia Medical University, Hohhot, Inner Mongolia, 010021, China.
Theriogenology. 2018 Jun;113:78-84. doi: 10.1016/j.theriogenology.2018.02.006. Epub 2018 Feb 9.
The survival rate of vitrified-thawed ovarian tissues after autotransplantation still needs to be improved. Therefore finding an ideal cryoprectant to reduce the damage to ovaries that caused by vitrification will pave the way for application of ovary cryopreservation on clinics. Experiments were conducted to investigate the effect of sodium alginate in cryoprotectant solution on mouse ovaries during the vitrification process. The ovaries obtained from 6-weeks old CD1 were assigned into six groups from A to F. Group A without treatment was used as the normal control. Group B cryopreserved with the basic cryoprotectant solution containing 15% each MeSO and EG was used as the experimental control. Groups C, D, E, and F cryopreserved with the basic cryoprotectant solution supplemented with 0.05%, 0.10%, 0.15%, and 0.20% of sodium alginate, respectively, were assigned for the experimental groups. The in vitro analyses showed that the developmental capability of the oocytes isolated from vitrified-thawed ovaries significantly increased with increasing concentration of sodium alginate in the cryoprotectant solution (groups: A = 70 ± 2; B = 43 ± 2; C = 48 ± 3; D = 53 ± 3; E = 60 ± 3; B < C < D < A, P < 0.05), and reached its highest level in group E with 0.15% of sodium alginate (P < 0.05). The lowest developmental capability of all groups was group F (41 ± 1%)(P < 0.05) with 0.20% of sodium alginate. The similar results were obtained by the autotransplantation in vivo. These finding demonstrated that sodium alginate can significantly reduce the damage to ovaries by vitrification.
玻璃化冷冻卵巢组织自体移植后的存活率仍有待提高。因此，寻找一种理想的冷冻保护剂以减少玻璃化对卵巢造成的损伤，将为卵巢冷冻保存技术在临床上的应用铺平道路。本实验旨在研究冷冻保护剂溶液中的海藻酸钠在玻璃化过程中对小鼠卵巢的影响。取6周龄CD1小鼠的卵巢，分为A至F六组。A组不做处理作为正常对照组。B组用含15%二甲基亚砜（MeSO）和15%乙二醇（EG）的基础冷冻保护剂溶液进行冷冻保存作为实验对照组。C、D、E、F组分别用添加了0.05%、0.10%、0.15%和0.20%海藻酸钠的基础冷冻保护剂溶液进行冷冻保存作为实验组。体外分析表明，从玻璃化冷冻解冻后的卵巢中分离出的卵母细胞的发育能力，随着冷冻保护剂溶液中海藻酸钠浓度的增加而显著提高（分组：A组=70±2；B组=43±2；C组=48±3；D组=53±3；E组=60±3；B＜C＜D＜A，P＜0.05），在添加0.15%海藻酸钠的E组达到最高水平（P＜0.05）。所有组中发育能力最低的是添加0.20%海藻酸钠的F组（41±1%）（P＜0.05）。体内自体移植也得到了类似结果。这些发现表明，海藻酸钠可显著减轻玻璃化对卵巢的损伤。
