Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark.
Cellular and Molecular Research Center, School of Medicine, Iran University of Medical Science, Tehran, Iran.
Biol Reprod. 2022 Nov 14;107(5):1177-1204. doi: 10.1093/biolre/ioac160.
Oogenesis and folliculogenesis are considered as complex and species-specific cellular differentiation processes, which depend on the in vivo ovarian follicular environment and endocrine cues. Considerable efforts have been devoted to driving the differentiation of female primordial germ cells toward mature oocytes outside of the body. The recent experimental attempts have laid stress on offering a suitable microenvironment to assist the in vitro folliculogenesis and oogenesis. Despite developing a variety of bioengineering techniques and generating functional mature gametes through in vitro oogenesis in earlier studies, we still lack knowledge of appropriate microenvironment conditions for building biomimetic culture systems for female fertility preservation. Therefore, this review paper can provide a source for a large body of scientists developing cutting-edge in vitro culture systems for female germ cells or setting up the next generation of reproductive medicine as feasible options for female infertility treatment. The focal point of this review outlines advanced bioengineering technologies such as 3D biofabricated hydrogels/scaffolds and microfluidic systems utilized with female germlines for fertility preservation through in vitro folliculogenesis and oogenesis.
卵子发生和卵泡发生被认为是复杂的、具有物种特异性的细胞分化过程，依赖于体内卵巢卵泡环境和内分泌信号。人们已经做出了相当大的努力来促使雌性原始生殖细胞在体外向成熟卵子分化。最近的实验尝试强调提供合适的微环境来辅助体外卵泡发生和卵子发生。尽管在早期研究中通过体外卵子发生开发了多种生物工程技术并生成了功能性成熟配子，但我们仍然缺乏构建用于女性生育力保存的仿生培养系统的合适微环境条件的知识。因此，这篇综述文章可以为大量致力于开发女性生殖细胞体外培养系统的科学家提供一个资源，或者为下一代生殖医学提供可行的选择，以治疗女性不孕。这篇综述的重点概述了先进的生物工程技术，如 3D 生物制造水凝胶/支架和微流控系统，这些技术与雌性生殖细胞一起用于通过体外卵泡发生和卵子发生来保存生育能力。
