Ayari-Fakhfakh Emna, Ghram Abdeljelil, Albina Emmanuel, Cêtre-Sossah Catherine
IRVT (Institut de la Recherche Vétérinaire de Tunisie), Tunis, Tunisie; Institut Pasteur de Tunis, Tunis, Tunisie; Université Tunis El Manar, Tunis, Tunisie; ASTRE, Univ Montpellier, CIRAD, INRA, Montpellier, France.
Institut Pasteur de Tunis, Tunis, Tunisie.
Vet Immunol Immunopathol. 2018 Mar;197:15-20. doi: 10.1016/j.vetimm.2018.01.001. Epub 2018 Jan 8.
The mosquito-borne Rift Valley fever virus (RVFV) causes severe diseases in domesticated animals including cattle, sheep, camels and goats. Capripoxviruses (CPV) are suitable vectors for multivalent vaccine development. A recombinant rKS1-based CPV expressing the gene encoding the viral glycoprotein Gn of RVFV has been shown to induce protection in mice and sheep. The aim of this study was to evaluate the immunogenicity induced by this candidate vaccine in goats, and the level of cytokines produced by RVFV-specific Th1 and Th2 lymphocytes. The results of this study suggest that Th2 mediates immunity mainly through the significant production of IL4, which, coupled with a decrease in IFN-γ, may be involved in the replication of the capripoxvirus expressing the G of RVFV. CD4+ cells may play the role of helper cells in B cell responses and neutralizing antibody production in the anti-CPV humoral response, leading to strong immunity against RVFV.
蚊媒传播的裂谷热病毒（RVFV）可在包括牛、羊、骆驼和山羊在内的家畜中引发严重疾病。山羊痘病毒（CPV）是开发多价疫苗的合适载体。一种基于rKS1的重组CPV，表达RVFV病毒糖蛋白Gn的编码基因，已被证明能在小鼠和绵羊中诱导产生保护作用。本研究的目的是评估这种候选疫苗在山羊中诱导的免疫原性，以及RVFV特异性Th1和Th2淋巴细胞产生的细胞因子水平。本研究结果表明，Th2主要通过大量产生IL4介导免疫，这与IFN-γ的减少相结合，可能参与了表达RVFV的G蛋白的山羊痘病毒的复制。CD4+细胞可能在抗CPV体液反应中的B细胞应答和中和抗体产生中发挥辅助细胞的作用，从而产生针对RVFV的强大免疫力。
