Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Mar Pollut Bull. 2018 Feb;127:453-457. doi: 10.1016/j.marpolbul.2017.12.015. Epub 2017 Dec 21.
Diuron is an alternative biocide suggested to replace organotin in formulating antifouling paints to be applied on water-going vessels hull. However, it is potentially harmful to various non-targeted marine organisms due to its toxic properties. Present study aimed to isolate, screen and identify the potential of Diuron-degrading bacteria collected from the marine sediments of Port Klang, Malaysia. Preliminary screening was conducted by exposing isolated bacteria to 430ng/L (background level), followed by 600ng/L and 1000ng/L of Diuron concentrations. Nine bacteria colonies survived the exposure of the above concentrations. However, only two strains can tolerate to survive up to 1000μg/L, which were then characterised and identified using phenotypic tests and the standard 16S rRNA molecular identification. The strains were identified as Comamonas jiangduensis SZZ 10 and Bacillus aerius SZZ 19 (GenBank accession numbers: KU942479 and KU942480, respectively). Both strains have the potential of Diuron biodegradation for future use.
敌草隆是一种替代生物杀灭剂的建议，以取代用于在水上船只船体上应用的防污涂料中的有机锡。然而，由于其毒性特性，它可能对各种非目标海洋生物造成危害。本研究旨在从马来西亚巴生港的海洋沉积物中分离、筛选和鉴定具有敌草隆降解能力的细菌。初步筛选是通过将分离的细菌暴露于 430ng/L（背景水平），然后暴露于 600ng/L 和 1000ng/L 的敌草隆浓度下进行的。有 9 个细菌菌落能在上述浓度下存活下来。然而，只有两种菌株能耐受高达 1000μg/L 的浓度，然后通过表型试验和标准 16S rRNA 分子鉴定对其进行了特征和鉴定。这两种菌株分别被鉴定为江都区丛毛单胞菌 SZZ 10 和 Aerius 芽孢杆菌 SZZ 19（GenBank 登录号：KU942479 和 KU942480）。这两种菌株都具有未来用于敌草隆生物降解的潜力。
