Liu Kai-Hui, Ding Xiao-Wei, Salam Nimaichand, Zhang Bo, Tang Xiao-Fei, Deng Baiwan, Li Wen-Jun
School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, 723001, Shaanxi, China.
State Key Laboratory of Biocontrol and Guandong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
Extremophiles. 2018 May;22(3):525-535. doi: 10.1007/s00792-018-1014-y. Epub 2018 Feb 23.
Fungal communities represent an indispensable part of the geothermal spring ecosystem; however, studies on fungal community within hot springs are still scant. Here, we used Illumina HiSeq 2500 sequencing to detect fungal community diversity in extremely acidic hot springs (pH < 4) and neutral and alkaline springs (pH > 6) of Tengchong-indicated by the presence of over 0.75 million valid reads. These sequences were phylogenetically assigned to 5 fungal phyla, 67 order, and 375 genera, indicating unexpected fungal diversity in the hot springs. The genera such as Penicillium, Entyloma, and Cladosporium dominated the fungal community in the acidic geothermal springs, while the groups such as Penicillium, Engyodontium, and Schizophyllum controlled the fungal assemblages in the alkaline hot springs. The alpha-diversity indices and the abundant fungal taxa were significantly correlated with physicochemical factors of the hot springs particularly pH, temperature, and concentrations of Fe, NH, NO and S, suggesting that the diversity and distribution of fungal assemblages can be influenced by the complex environmental factors of hot springs.
真菌群落是温泉生态系统不可或缺的一部分；然而，关于温泉内部真菌群落的研究仍然很少。在此，我们使用Illumina HiSeq 2500测序技术，检测了腾冲极酸性温泉（pH < 4）以及中性和碱性温泉（pH > 6）中的真菌群落多样性——超过75万个有效读数表明了这一点。这些序列在系统发育上被归类为5个真菌门、67个目和375个属，表明温泉中存在意想不到的真菌多样性。青霉属、叶瘤菌属和枝孢属等属在酸性地热温泉的真菌群落中占主导地位，而青霉属、内齿菌属和裂褶菌属等类群则控制着碱性温泉中的真菌组合。α多样性指数和丰富的真菌分类群与温泉的物理化学因素显著相关，尤其是pH值、温度以及铁、铵、硝酸盐和硫的浓度，这表明真菌组合的多样性和分布可能受到温泉复杂环境因素的影响。
