多参数流式细胞术在神经炎症和神经退行性变过程中研究中枢神经系统小胶质细胞和巨噬细胞异质性的应用。

Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Central Nervous System During Neuroinflammation and Neurodegeneration.

作者信息

Dukhinova Marina, Kopeikina Ekaterina, Ponomarev Eugene D

机构信息

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, China.

出版信息

Methods Mol Biol. 2018;1745:167-177. doi: 10.1007/978-1-4939-7680-5_10.

DOI:10.1007/978-1-4939-7680-5_10

PMID:29476469

Abstract

The resident macrophages of the central nervous system (CNS), also known as microglia, and blood-derived macrophages play an important role in the functional activity of the normal CNS, as well as in the development of neuroinflammation during various neurodegenerative disorders. Microglia and macrophages represent heterogeneous populations, which can modulate CNS environment and have different effects on neuronal regeneration. In this chapter, the main features of microglial and macrophage subsets and current methods for investigation of their heterogeneity will be discussed.

摘要

中枢神经系统（CNS）的常驻巨噬细胞，也称为小胶质细胞，以及源自血液的巨噬细胞，在正常中枢神经系统的功能活动以及各种神经退行性疾病期间神经炎症的发展中发挥着重要作用。小胶质细胞和巨噬细胞代表异质性群体，它们可以调节中枢神经系统环境并对神经元再生产生不同影响。在本章中，将讨论小胶质细胞和巨噬细胞亚群的主要特征以及目前研究它们异质性的方法。