School of Biological Sciences, University of Western Australia, Perth, WA, 6009, Australia.
Institute of Agriculture, University of Western Australia, Perth, WA, 6009, Australia.
Plant Cell Environ. 2018 Jul;41(7):1512-1523. doi: 10.1111/pce.13179. Epub 2018 May 10.
To study mechanism underpinning the calcifuge habit of some Lupinus species, especially under low-phosphorus (P) conditions, Lupinus species that were likely to respond differently to calcium (Ca) availability were assembled, and the sensitivity to Ca under a low-P supply was assessed. Seven Lupinus species (9 genotypes, L. albus L. cv Kiev, L. albus L. P26766, L. angustifolius L. cv Mandelup, L. angustifolius L. P26723, L. luteus L. cv Pootalong, L. hispanicus ssp. bicolor Boiss. and Reut. P22999, L. pilosus Murr. P27440, L. cosentinii Guss. P27225, and L. atlanticus Gladst. P27219) were grown hydroponically at 10 or 6000 μM Ca. Leaf symptoms, gas exchange and biomass were recorded; leaf and root nutrient concentrations were analysed, and the leaf cell types in which Ca and P accumulated were determined using elemental X-ray microanalyses. Calcium toxicity was demonstrated for L. angustifolius P26723, L. hispanicus ssp. bicolor. P22999, and L. cosentinii P27225, whereas the other species were tolerant of a high Ca supply under low-P conditions. In addition, genotypic differences in Ca toxicity were found within L. angustifolius. Most Ca accumulated in the mesophyll cells in all species, whereas most P was located in epidermal cells.
为了研究某些羽扇豆属植物（尤其是在低磷条件下）形成非钙养植物特性的机制，选择了一些可能对钙供应有不同响应的羽扇豆属植物，并评估了它们在低磷供应条件下对钙的敏感性。选择了 7 种羽扇豆属植物（9 个基因型，包括白羽扇豆 L. albus L. cv Kiev、白羽扇豆 L. albus L. P26766、窄叶羽扇豆 L. angustifolius L. cv Mandelup、窄叶羽扇豆 L. angustifolius L. P26723、黄羽扇豆 L. luteus L. cv Pootalong、双色二型蓝花棘豆 L. hispanicus ssp. bicolor Boiss. and Reut. P22999、多毛棘豆 L. pilosus Murr. P27440、高山蓝花棘豆 L. cosentinii Guss. P27225 和大西洋羽扇豆 L. atlanticus Gladst. P27219），在 10 或 6000 μM Ca 条件下进行水培种植。记录叶片症状、气体交换和生物量；分析叶片和根系养分浓度，并使用元素 X 射线微分析确定 Ca 和 P 积累的叶片细胞类型。窄叶羽扇豆 P26723、双色二型蓝花棘豆 P22999 和高山蓝花棘豆 P27225 表现出 Ca 毒性，而其他物种在低磷条件下对高 Ca 供应具有耐受性。此外，在窄叶羽扇豆属内发现了 Ca 毒性的基因型差异。所有物种中，大部分 Ca 积累在叶肉细胞中，而大部分 P 则位于表皮细胞中。
