Escobar Enrico, Gómez-Valenzuela Fernán, Peñafiel Cristian, Chimenos-Küstner Eduardo, Pérez-Tomás Ricardo
Department of Oral Pathology and Medicine, Faculty of Dentistry, University of Chile, Santiago, Chile.
Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.
J Clin Exp Dent. 2023 Feb 1;15(2):e125-e134. doi: 10.4317/jced.59769. eCollection 2023 Feb.
The growth of ameloblastomas (odontogenic tumours) and odontogenic keratocyst (OKC) (developmental cyst) is associated with the expression of proteins related to cell survival and apoptosis. Bcl-2-associated protein X (Bax) and the tumour suppressor protein p53 collectively promote p53-mediated apoptosis. This study aimed to assess the immunohistochemical expression of p53, Bcl-2 and Bax in conventional ameloblastoma (CA), unicystic ameloblastoma (UA) types, and OKC sporadic (OKC-NS/S) and syndromic (OKC-NBSCC).
Paraffinized blocks of CA (n=18), UA (n=15), OKC-NS/S (n=18) and OKC-NBSCC (n=15) fixed in 10% formalin were used. After diagnosis, tissue specimens were stained by immunohistochemistry for p53, Bcl-2 and Bax marker. Stained cells were randomly counted in five high power fields. The data analysis was performed via Shapiro-Wilk test, ANOVA with Tukey's multiple comparisons or Kruskal-Wallis with Dunn's multiple comparisons. Statistical significance was defined as <0.05.
We did not observe differences between p53 expression in CA, mural UA (MUA), intraluminal/luminal UA (I/LUA), OKC-NS/S, and OKC-NBSCC (19.69%, 18.74%, 16.76%, 12.35% and 9.04%, respectively). Similar results were recognized for Bax expression in CA, MUA, I/LUA, OKC-NS/S, and OKC-NBSCC (33.72%, 34.95%, 22.94, 21.58% and 20.76%, respectively). However, we recognized significant differences between Bcl-2 expression in OKC-NS/S vs MUA, OKC-NS/S vs I/LUA, OKC-NS/S vs CA, OKC-NBSCC vs MUA, OKC-NBSCC vs I/LUA, and I/LUA vs CA. P53, Bcl-2 and Bax levels were higher in mural morphological areas versus intraluminal and luminal morphological areas in UA.
There is a tendency for an increased expression of p53, Bcl-2, and Bax proteins in CA, and mural proliferation of UA, compared to lesions with a cystic morphology, which could be associated with a local aggressive behaviour. p53, Bcl-2, Bax protein, apoptosis, odontogenic tumour, odontogenic cyst.
成釉细胞瘤（牙源性肿瘤）和牙源性角化囊肿（OKC，发育性囊肿）的生长与细胞存活和凋亡相关蛋白的表达有关。Bcl-2相关蛋白X（Bax）和肿瘤抑制蛋白p53共同促进p53介导的凋亡。本研究旨在评估p53、Bcl-2和Bax在传统型成釉细胞瘤（CA）、单囊性成釉细胞瘤（UA）、散发性OKC（OKC-NS/S）和综合征性OKC（OKC-NBSCC）中的免疫组化表达。
使用固定于10%福尔马林中的CA（n=18）、UA（n=15）、OKC-NS/S（n=18）和OKC-NBSCC（n=15）的石蜡块。诊断后，组织标本通过免疫组化检测p53、Bcl-2和Bax标记物。在五个高倍视野中随机计数染色细胞。数据分析通过Shapiro-Wilk检验、Tukey多重比较的方差分析或Dunn多重比较的Kruskal-Wallis检验进行。统计学显著性定义为<0.05。
我们未观察到CA、壁性UA（MUA）、腔内/管腔内UA（I/LUA）、OKC-NS/S和OKC-NBSCC中p53表达的差异（分别为19.69%、18.74%、16.76%、12.35%和9.04%）。CA、MUA、I/LUA、OKC-NS/S和OKC-NBSCC中Bax表达也得到类似结果（分别为33.72%、34.95%、22.94%、21.58%和20.76%）。然而，我们发现OKC-NS/S与MUA、OKC-NS/S与I/LUA、OKC-NS/S与CA、OKC-NBSCC与MUA、OKC-NBSCC与I/LUA以及I/LUA与CA之间Bcl-2表达存在显著差异。UA中壁性形态区域的p53、Bcl-2和Bax水平高于腔内和管腔内形态区域。
与具有囊性形态的病变相比，CA和UA的壁性增殖中p53、Bcl-2和Bax蛋白表达有增加的趋势，这可能与局部侵袭性行为有关。p53、Bcl-2、Bax蛋白、凋亡、牙源性肿瘤、牙源性囊肿。
