Hospital Israelita Albert Einstein - Avenida Albert Einstein, 627, Morumbi, CEP: 05652-900, Sao Paulo, Brazil.
Department of Obstetrics and Gynecology, University of Sao Paulo Medical School - Av. Dr. Enéas de Carvalho Aguiar, 255, Cerqueira César, CEP: 05403-000, Sao Paulo, Brazil.
J Reprod Immunol. 2018 Apr;126:32-38. doi: 10.1016/j.jri.2018.02.003. Epub 2018 Feb 6.
The aim of this study was to evaluate Treg and NK cells related cytokines in deep infiltrating endometriosis lesions and its relationship with clinical symptoms of the disease. mRNA expression of Transforming Growth Factor Beta (TGFB), Interleukin (IL)10, Interferon Gamma (IFNG), IL7, and IL15 was analyzed by Real-Time PCR in eutopic endometrium and rectosigmoid lesions from 11 women with deep infiltrating endometriosis and in eutopic endometrium from 11 healthy women. IL10, IFNG, and IL7 expression was significantly higher in endometriotic bowel lesions than in eutopic endometrium from women with endometriosis. IL10 and TGFB expression was significantly higher in endometriotic bowel lesions than in eutopic endometrium from healthy women. In addition, TGFB and IL15 levels correlated positively with deep dyspareunia and cyclic dyschezia, respectively, while IL7 levels correlated negatively with dysmenorrhea. Deep infiltrating rectosigmoid endometriosis displays alterations in Treg and NK cells related cytokine, and TGFB, IL7 and IL15 expression is related with dyspareunia, dysmenorrhea and cyclic dyschezia, respectively, in patients with the disease.
本研究旨在评估深浸润型子宫内膜异位症病灶中调节性 T 细胞（Treg）和自然杀伤（NK）细胞相关细胞因子及其与疾病临床症状的关系。采用实时 PCR 法分析 11 例深浸润型子宫内膜异位症患者在位子宫内膜和直肠乙状结肠病灶及 11 例健康女性在位子宫内膜中转化生长因子-β（TGFB）、白细胞介素（IL）10、干扰素-γ（IFNG）、IL7 和 IL15 的 mRNA 表达。与子宫内膜异位症患者的在位子宫内膜相比，子宫内膜异位症患者的直肠乙状结肠病灶中 IL10、IFNG 和 IL7 的表达显著升高。与健康女性的在位子宫内膜相比，子宫内膜异位症患者的直肠乙状结肠病灶中 IL10 和 TGFB 的表达显著升高。此外，TGFB 水平与深部性交痛呈正相关，IL15 水平与周期性排便困难呈正相关，而 IL7 水平与痛经呈负相关。深浸润型直肠乙状结肠子宫内膜异位症显示出 Treg 和 NK 细胞相关细胞因子的改变，TGFB、IL7 和 IL15 的表达与疾病患者的性交痛、痛经和周期性排便困难分别相关。
