Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China.
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China; Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
Peptides. 2019 Dec;122:169929. doi: 10.1016/j.peptides.2018.02.007. Epub 2018 Mar 15.
Insects must undergo ecdysis for successful development and growth, and the crustacean cardioactive peptide (CCAP) is one of the most important hormone in this process. Here we reported a cDNA encoding for the CCAP precursor cloned from the oriental fruit fly, Bactrocera dorsalis, a most destructive insect pest of agriculture. The CCAP mature peptide (PFCNAFTGC-NH2) of B. dorsalis was generated by post-translational processing and found to be highly comparable with other insects. RT-qPCR showed that mRNA of CCAP in B. dorsalis (BdCCAP) was predominantly expressed in the central nervous system (CNS) and midgut of 3rd-instar larvae. By using immunohistochemical analysis, we also localized the endocrine cells that produce CCAP in the CNS, ring gland and midgut of 3rd-instar larvae of B. dorsalis. The synthetic CCAP mature peptide could induce the expression of mRNA of adipokinetic hormone (AKH), the metabolic neuropeptides in insects. The expression of BdCCAP mRNA in the CNS, but not in the midgut, could be upregulated in the response to the challenge of insect molting hormone, 20-hydroxyecdysone.
昆虫必须经历蜕皮才能成功发育和生长，而甲壳动物心脏活性肽 (CCAP) 是这个过程中最重要的激素之一。在这里，我们报道了一种从东方果实蝇 Bactrocera dorsalis 中克隆的 CCAP 前体的 cDNA，B. dorsalis 是农业上最具破坏性的害虫之一。B. dorsalis 的 CCAP 成熟肽 (PFCNAFTGC-NH2) 通过翻译后加工生成，与其他昆虫高度相似。RT-qPCR 显示，B. dorsalis（BdCCAP）中 CCAP 的 mRNA 主要在中枢神经系统 (CNS) 和 3 龄幼虫的中肠中表达。通过免疫组织化学分析，我们还定位了产生 CCAP 的内分泌细胞在 CNS、环腺和 3 龄幼虫的中肠中。合成的 CCAP 成熟肽可以诱导昆虫代谢神经肽——脂肪激素 (AKH) 的 mRNA 表达。在对昆虫蜕皮激素 20-羟基蜕皮酮的挑战中，CNS 中的 BdCCAP mRNA 表达而非中肠中的表达可以上调。
