Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China; International China-Belgium Joint Laboratory on Sustainable Crop Pest Control Between Southwest University in China and Ghent University in Belgium, Chongqing, 400715, China.
International China-Belgium Joint Laboratory on Sustainable Crop Pest Control Between Southwest University in China and Ghent University in Belgium, Chongqing, 400715, China; Department of Plants and Crops, Ghent University, Ghent, 9000, Belgium.
Insect Biochem Mol Biol. 2020 Dec;127:103475. doi: 10.1016/j.ibmb.2020.103475. Epub 2020 Oct 13.
MicroRNAs (miRNAs) are endogenous small noncoding RNAs (18-25 nt) that are involved in many physiological processes including development, cancer, immunity, apoptosis and host-microbe interactions through post-transcriptional regulation of gene expression. In this study, we measured the profile of small RNAs over the developmental transitions of the oriental fruit fly Bactrocera dorsalis from egg hatching, molting, and pupation to adult eclosion. We identified 250 miRNAs, including 83 known and 167 novel miRNAs, and 47 isomiRNAs. In addition, we identified the miRNAs differentially expressed over the developmental transitions. Interestingly, the miR-309 cluster, the miR-2 cluster/family and the let-7 cluster were among these differentially expressed miRNAs, suggesting a role in the regulation of egg hatching, molting and pupation/adult eclosion, respectively. Moreover, a detailed analysis of the temporal expression patterns of 14 highly expressed miRNAs in the pupal stage revealed three types of expression profiles. Furthermore, injection of a miR-100 mimic in the 3rd instar larvae resulted in a significant decrease in pupation and adult eclosion rates, whereas injection of a miR-317 antagomir resulted in a significant decrease in the pupation rate and a decrease in the pupation time, indicating that miR-100 and miR-317 are involved in the process of pupation. Finally, injection of a miR-100/miR-285 mimic or antagomir in pupae resulted in a significant decrease in the eclosion rate and a significant increase in the prevalence of a partial eclosion phenotype, implying the involvement of miR-100 and miR-285 in the process of adult eclosion. This study identified critical miRNAs involved in the transitions of this important holometabolic model and pest insect B. dorsalis from egg hatching to adult eclosion, thus providing a useful resource for exploring the regulatory role of miRNAs during insect post-embryonic development.
微小 RNA（miRNAs）是内源性的小非编码 RNA（18-25nt），通过对基因表达的转录后调控，参与包括发育、癌症、免疫、凋亡和宿主-微生物相互作用在内的许多生理过程。在这项研究中，我们测量了东方果实蝇 Bactrocera dorsalis 从卵孵化、蜕皮、化蛹到成虫羽化的发育过渡过程中小 RNA 的谱。我们鉴定了 250 个 miRNAs，包括 83 个已知的和 167 个新的 miRNAs，以及 47 个同工型 miRNA。此外，我们还鉴定了在发育过渡过程中差异表达的 miRNAs。有趣的是，miR-309 簇、miR-2 簇/家族和 let-7 簇都在这些差异表达的 miRNAs 中，这表明它们在调节卵孵化、蜕皮和化蛹/成虫羽化方面发挥作用。此外，对蛹期 14 个高表达 miRNAs 的时间表达模式进行详细分析，揭示了三种表达谱类型。此外，在 3 龄幼虫中注射 miR-100 模拟物会导致化蛹和成虫羽化率显著降低，而注射 miR-317 拮抗剂会导致化蛹率显著降低，并缩短化蛹时间，表明 miR-100 和 miR-317 参与化蛹过程。最后，在蛹期注射 miR-100/miR-285 模拟物或拮抗剂会导致羽化率显著降低，部分羽化表型的发生率显著增加，表明 miR-100 和 miR-285 参与成虫羽化过程。本研究鉴定了在这个重要的全变态模式生物和害虫东方果实蝇从卵孵化到成虫羽化的过渡过程中涉及的关键 miRNAs，为探索 miRNA 在昆虫胚胎后发育过程中的调控作用提供了有用的资源。
