Department of Entomology, South China Agricultural University, Guangzhou, China.
Department of Entomology, South China Agricultural University, Guangzhou, China
Appl Environ Microbiol. 2019 Jun 17;85(13). doi: 10.1128/AEM.00803-19. Print 2019 Jul 1.
The insect microbiota can change dramatically to enable adaptation of the host in different developmental stages and environments; however, little is known about how the host maintains its microbiota to achieve such adaptations. In this study, 16S rRNA sequencing revealed that the microorganisms in larvae and adults of the Oriental fruit fly, , are primarily Gram-negative bacteria but that the major components in pupae are Gram-positive bacteria. Using suppression subtractive hybridization (SSH) and transcriptome analysis, we screened two specifically expressed genes encoding peptidoglycan recognition proteins (PGRP-LB and PGRP-SB1) and analyzed their relationship to microbial communities. Knockdown of the PGRP-LB gene in larvae and adults led to increased ratios of Gram-positive bacteria; knockdown of the PGRP-SB1 gene in pupae led to increased ratios of Gram-negative bacteria. Our results suggest that maintenance of the microbiota in different developmental stages of may be associated with the PGRP-LB and PGRP-SB1 genes. Microorganisms are ubiquitous in insects and have widespread impacts on multiple aspects of insect biology. However, the microorganisms present in insects can change dramatically in different developmental stages, and it is critical to maintain the appropriate microorganisms in specific host developmental stages. Therefore, analysis of the factors associated with the microbiota in specific development stages of the host is needed. In this study, we applied suppression subtractive hybridization (SSH) combined with transcriptome analysis to investigate whether the microbiota in development stages of the Oriental fruit fly, , is associated with expression of PGRP genes. We found that two different PGRP genes were specifically expressed during development and that these genes may be associated with changes in microbial communities in different developmental stages of .
昆虫微生物组可以发生显著变化，使宿主在不同的发育阶段和环境中适应；然而，人们对宿主如何维持其微生物组以实现这种适应知之甚少。在这项研究中，16S rRNA 测序显示，东方果实蝇幼虫和成虫的微生物主要是革兰氏阴性菌，但蛹的主要成分是革兰氏阳性菌。利用抑制性消减杂交（SSH）和转录组分析，我们筛选了两个特异性表达的编码肽聚糖识别蛋白（PGRP-LB 和 PGRP-SB1）的基因，并分析了它们与微生物群落的关系。幼虫和成虫中 PGRP-LB 基因的敲低导致革兰氏阳性菌比例增加；蛹中 PGRP-SB1 基因的敲低导致革兰氏阴性菌比例增加。我们的结果表明，维持 不同发育阶段的微生物组可能与 PGRP-LB 和 PGRP-SB1 基因有关。微生物在昆虫中无处不在，对昆虫生物学的多个方面都有广泛的影响。然而，昆虫体内的微生物在不同的发育阶段可以发生显著变化，在特定的宿主发育阶段维持适当的微生物是至关重要的。因此，需要分析与宿主特定发育阶段的微生物组相关的因素。在这项研究中，我们应用抑制性消减杂交（SSH）结合转录组分析来研究东方果实蝇发育阶段的微生物组是否与 PGRP 基因的表达有关。我们发现两个不同的 PGRP 基因在发育过程中特异性表达，这些基因可能与不同发育阶段微生物群落的变化有关。
