Enzymology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
College of Applied Medical Sciences, Qassim University, Buraidah, Saudi Arabia.
Int J Biol Macromol. 2018 Jul 1;113:300-308. doi: 10.1016/j.ijbiomac.2018.02.098. Epub 2018 Feb 22.
The study of drug-DNA interactions is of great importance, as it paves the way towards the design of better therapeutic agents. Here, the interaction of DNA with a therapeutic and prophylactic drug citral has been studied. We have attempted to ascertain the mode of binding of citral with calf thymus DNA (Ct-DNA) through various biophysical techniques. Analysis of the UV-visible absorbance spectra and fluorescence spectra indicated the formation of a complex between citral and Ct-DNA. Competitive binding assays with ethidium bromide (EB), acridine orange (AO) and Hoechst 33258 reflected that citral possibly intercalates within the Ct-DNA. These observations were further confirmed by circular dichroism (CD) spectral analysis, viscosity measurements, DNA melting and molecular docking studies. This study is expected to contribute to a better understanding of molecular mechanisms of citral, and design of new drugs in the future.
药物-DNA 相互作用的研究具有重要意义，因为它为设计更好的治疗药物铺平了道路。在这里，研究了治疗和预防药物柠檬醛与 DNA 的相互作用。我们试图通过各种物理化学技术确定柠檬醛与小牛胸腺 DNA（Ct-DNA）的结合方式。紫外可见吸收光谱和荧光光谱分析表明，柠檬醛与 Ct-DNA 形成了复合物。与溴化乙锭（EB）、吖啶橙（AO）和 Hoechst 33258 的竞争结合实验表明，柠檬醛可能嵌入 Ct-DNA 中。圆二色性（CD）光谱分析、粘度测量、DNA 热融和分子对接研究进一步证实了这一点。这项研究有望增进对柠檬醛的分子机制的理解，并为未来设计新药做出贡献。
