Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 300, Taiwan, ROC.
Department of Biomedical Engineering, National Yang Ming University, Taipei 112, Taiwan, ROC.
Biosens Bioelectron. 2018 Jun 1;107:259-265. doi: 10.1016/j.bios.2018.02.036. Epub 2018 Feb 15.
Continued risk assessment by evaluating cardiac biomarkers in healthy and unhealthy individuals can lower the mortality rate of cardiovascular diseases (CVDs). In this research, we have developed a hand-held biosensor system to rapidly screen for brain natriuretic peptide (BNP) from a single drop of whole blood. The sensor methodology is based on extended gate design of electrical double layer (EDL) field effect transistor (FET), that can directly detect BNP in whole blood, without extensive sample pre-treatments, thereby eliminating the limitations of charge screening in high ionic strength solutions. A simple sensor array chip is fabricated to integrate with the MOSFET sensor system. Sensing characteristics are elucidated using purified BNP samples in 1 × PBS (with 4% BSA), spiked BNP samples in whole blood and clinical whole blood samples. The blood cells can be gravitationally separated without the use of any external actuation. The sensor exhibits very high sensitivity over wide dynamic range of detection. The sensing characteristics are not adversely affected by the presence of background proteins or blood cells, even without gravitational blood cell separation. Thus, the biosensor system can allow users to perform rapid whole blood diagnostics with minimal user protocols, in 5 min. The features of high sensitivity, cost-effectiveness and convenience of usage empower this technology to revolutionize the mobile diagnostics and healthcare industry.
通过评估健康和不健康个体中的心脏生物标志物来持续进行风险评估，可以降低心血管疾病 (CVD) 的死亡率。在这项研究中，我们开发了一种手持式生物传感器系统，可从单个全血样本中快速筛选脑钠肽 (BNP)。该传感器方法基于电双层 (EDL) 场效应晶体管 (FET) 的扩展门设计，可直接在全血中检测 BNP，无需进行广泛的样品预处理，从而消除了高离子强度溶液中电荷筛选的限制。制作了一个简单的传感器阵列芯片以与 MOSFET 传感器系统集成。使用 1×PBS（含 4%BSA）中的纯化 BNP 样品、全血中的掺杂 BNP 样品和临床全血样品来阐明传感特性。无需任何外部致动即可通过重力分离血细胞。该传感器在宽检测动态范围内表现出非常高的灵敏度。即使没有重力血细胞分离，传感器的特性也不会受到背景蛋白或血细胞的不利影响。因此，该生物传感器系统可以允许用户在 5 分钟内通过最小的用户协议进行快速的全血诊断。该技术具有高灵敏度、成本效益和使用方便的特点，有望彻底改变移动诊断和医疗保健行业。
