Liao Liang-Wen, Chen Po-Hsuan, Tsai Shu-Yi, Tripathi Adarsh, Paulose Akhil K, Chang Shing-Jyh, Wang Yu-Lin
Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Institute of Molecular Medicine, National Tsing Hua University, Hsinchu 30013, Taiwan.
Biomicrofluidics. 2021 Apr 5;15(2):024106. doi: 10.1063/5.0042522. eCollection 2021 Mar.
In this experimental study, a portable biosensor was developed to detect β-human chorionic gonadotropin (β-hCG), which is extensively used in pregnancy tests and serves as a biomarker for ectopic pregnancy. The sensor used is an electric-double-layer field-effect transistor biosensor with the extended-gate design. Bias voltage is applied on the sensor to measure the resulting drain current signals. Gold electrode surface is functionally activated with an anti-β-hCG antibody to capture β-hCG protein. Fluorescence imaging technique is utilized to confirm the surface functionalization. The biosensor demonstrates a dynamically wide range of molecules as detection targets at very low sample concentrations, which shows the potential to detect ectopic pregnancy in very early stages and easily keep track of its periodic changes. It can be produced en masse and does not use additional labels/reagents or pre-processing techniques for the sample. This biosensor can significantly reduce the manufacturing costs and is comparable with the currently available commercial ß-hCG assays. It is suitable for early diagnosis of ectopic pregnancy with low cost and easy operation at home with urine samples.
在这项实验研究中，开发了一种便携式生物传感器来检测β-人绒毛膜促性腺激素（β-hCG），β-hCG广泛用于妊娠试验，并作为异位妊娠的生物标志物。所使用的传感器是具有扩展栅设计的双电层场效应晶体管生物传感器。在传感器上施加偏置电压以测量产生的漏极电流信号。金电极表面用抗β-hCG抗体进行功能活化，以捕获β-hCG蛋白。利用荧光成像技术确认表面功能化。该生物传感器在非常低的样品浓度下能动态检测多种分子作为检测目标，显示出在极早期检测异位妊娠并轻松跟踪其周期性变化的潜力。它可以大规模生产，并且不使用额外的标记物/试剂或样品预处理技术。这种生物传感器可以显著降低制造成本，并且与目前可用的商业β-hCG检测方法相当。它适用于以低成本在家中使用尿液样本对异位妊娠进行早期诊断，操作简便。
