Ranjitha Dhanasekaran A, Gardiner Katheleen J
* Rocky Mountain Alzheimer's Disease Center, School of Medicine, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado 80045, USA.
† Department of Neurology, School of Medicine, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado 80045, USA.
J Bioinform Comput Biol. 2018 Jun;16(3):1850001. doi: 10.1142/S0219720018500014. Epub 2018 Jan 15.
Reverse Phase Protein Arrays (RPPA) is a high-throughput technology used to profile levels of protein expression. Handling the large datasets generated by RPPA can be facilitated by appropriate software tools. Here, we describe RPPAware, a free and intuitive software suite that was developed specifically for analysis and visualization of RPPA data. RPPAware is a portable tool that requires no installation and was built using Java. Many modules of the tool invoke R to utilize the statistical features. To demonstrate the utility of RPPAware, data generated from screening brain regions of a mouse model of Down syndrome with 62 antibodies were used as a case study. The ease of use and efficiency of RPPAware can accelerate data analysis to facilitate biological discovery. RPPAware 1.0 is freely available under GNU General Public License from the project website at http://downsyndrome.ucdenver.edu/iddrc/rppaware/home.htm along with a full documentation of the tool.
反向蛋白质阵列（RPPA）是一种用于分析蛋白质表达水平的高通量技术。合适的软件工具有助于处理RPPA产生的大量数据集。在此，我们介绍RPPAware，这是一款专门为RPPA数据的分析和可视化而开发的免费且直观的软件套件。RPPAware是一个无需安装的便携式工具，用Java构建。该工具的许多模块调用R语言以利用其统计功能。为了证明RPPAware的实用性，我们将使用62种抗体对唐氏综合征小鼠模型的脑区进行筛选所产生的数据作为案例研究。RPPAware的易用性和高效性能够加速数据分析，以促进生物学发现。RPPAware 1.0可根据GNU通用公共许可证从项目网站http://downsyndrome.ucdenver.edu/iddrc/rppaware/home.htm免费获取，同时还提供该工具的完整文档。
