Cardiovascular Research Center, University of Michigan, Ann Arbor, MI, USA.
Pharmaceutical and Health Sciences Department, Faculty of Pharmacy, Universidad San Pablo-CEU Universities, Boadilla del Monte, Madrid, Spain.
Food Chem. 2018 Jun 30;252:142-146. doi: 10.1016/j.foodchem.2018.01.084. Epub 2018 Jan 12.
The inhibitory potential of Montmorency tart cherry on glycemia regulation and other enzymes relevant to inflammation were evaluated. Tart cherry has superior inhibitory potential against key enzymes associated with carbohydrate digestion linked to hypertension. In particular, α-amylase activity was significantly inhibited (IC50 = 3.46 ± 0.06 mg/ml), whereas we observed mild inhibition of α-glucosidase (IC50 = 11.64 ± 0.65 mg/ml). Angiotensin I-converting enzyme inhibition was also strong by about 89%. Tart cherry extract showed strong to moderate inhibitions of cyclooxygenase-1 (65%), lipoxygenase (64%), cyclooxygenase-2 (38%) and xanthine oxidase (26%), respectively. Anthocyanins, cyanidin 3-rutinoside and cyanidin 3-glucoside, were strong inhibitors of α-amylase and α-glucosidase. Kaempferol showed relatively potent inhibition on COX and XO. It was revealed that some pairs of metabolites manifest positive or negative interactions against XO enzyme inhibition. Inhibition of all these enzymes provides a strong biochemical basis for management of type 2 diabetes and cardiovascular disease by controlling glucose absorption, reducing associated hypertension and inflammation.
评估了蒙莫朗西酸樱桃对血糖调节和其他与炎症相关的酶的抑制潜力。酸樱桃对与高血压相关的碳水化合物消化相关的关键酶具有卓越的抑制潜力。特别是，α-淀粉酶活性受到显著抑制（IC50=3.46±0.06mg/ml），而我们观察到对α-葡萄糖苷酶的抑制作用较弱（IC50=11.64±0.65mg/ml）。血管紧张素 I 转换酶抑制作用也很强，约为 89%。酸樱桃提取物对环加氧酶-1（65%）、脂加氧酶（64%）、环加氧酶-2（38%）和黄嘌呤氧化酶（26%）的抑制作用分别为强至中度。花色苷、矢车菊素 3-芸香糖苷和矢车菊素 3-葡萄糖苷是α-淀粉酶和α-葡萄糖苷酶的强抑制剂。山奈酚对 COX 和 XO 具有相对较强的抑制作用。结果表明，一些代谢物对 XO 酶抑制的相互作用呈阳性或阴性。这些酶的抑制作用为通过控制葡萄糖吸收、降低相关高血压和炎症来管理 2 型糖尿病和心血管疾病提供了强有力的生化基础。
