硫辛酸对小鼠抗体反应的增强作用。II. 免疫抑制小鼠抗体反应的恢复

Augmentation of the antibody response by lipoic acid in mice. II. Restoration of the antibody response in immunosuppressed mice.

作者信息

Ohmori H, Yamauchi T, Yamamoto I

出版信息

Jpn J Pharmacol. 1986 Oct;42(2):275-80. doi: 10.1254/jjp.42.275.

DOI:10.1254/jjp.42.275

Abstract

Lipoic acid (Lip) was examined for its effect on the in vivo antibody response in mice. It was found that Lip significantly restored the suppressed antibody response to sheep erythrocytes (SRBC) in cyclophosphamide-injected mice when it was orally administered at 25 mg/kg twice a day for 4 consecutive days after immunization. On the other hand, Lip-administration did not affect the antibody response in normal mice immunized with SRBC. Normal or cyclophosphamide-treated mice were primed with horse erythrocytes (HRBC) and were given either saline or Lip for 3 consecutive days. HRBC-specific helper T cell activity of their spleen cells were compared by coculturing these cells with trinitrophenyl (TNP)-keyhole limpet hemocyanin-primed spleen cells in the presence of TNP-HRBC as the antigen. A significant restoration of the suppressed helper T cell activity was observed in the Lip-administered group. However, the helper T cell activity was not affected significantly by Lip-administration in normal mice. Lip could also partially restore the helper T cell activities that were suppressed by the treatment with hydrocortisone or X-ray irradiation.

摘要

研究了硫辛酸（Lip）对小鼠体内抗体反应的影响。结果发现，在免疫后连续4天每天两次口服25mg/kg的Lip，可显著恢复环磷酰胺注射小鼠对绵羊红细胞（SRBC）受抑制的抗体反应。另一方面，给予Lip并不影响用SRBC免疫的正常小鼠的抗体反应。用马红细胞（HRBC）对正常或环磷酰胺处理的小鼠进行致敏，并连续3天给予生理盐水或Lip。通过将这些细胞与用三硝基苯基（TNP）-匙孔血蓝蛋白致敏的脾细胞在存在TNP-HRBC作为抗原的情况下共培养，比较它们脾细胞的HRBC特异性辅助性T细胞活性。在给予Lip的组中观察到受抑制的辅助性T细胞活性显著恢复。然而，给予Lip对正常小鼠的辅助性T细胞活性没有显著影响。Lip还可以部分恢复因氢化可的松或X射线照射处理而受抑制的辅助性T细胞活性。