Oral Pathology and Medicine Postgraduate Program, Universidad Autónoma Metropolitana, Mexico City, Mexico.
Biomedical Research Unit, Instituto Nacional de Cancerología (INCan)/Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México City, Mexico.
Oral Dis. 2018 Mar;24(1-2):210-214. doi: 10.1111/odi.12732.
Although HPV emerged as a crucial carcinogenic and prognostic biomarker in head and neck cancer, and considering the increase in HPV-associated oral lesions (HPV-OLs) in HIV individuals, molecular information about HPV-OLs is scarce; thus, our aim was to determine viral loads in HPV-OLs from HIV/AIDS individuals.
HIV/AIDS subjects with HPV-OL were included in this cross-sectional study. Following informed consent, biopsies were obtained. HPV detection and typing were carried out by PCR and sequencing (MY09/11, GP5+/6+). HPV-13 and HPV-32 loads were determined by a high-resolution melting assay. For statistical analysis, X , Fisher's exact, and Mann-Whitney U tests were applied, using SPSS software (v.23).
Twenty-nine HIV subjects (median age 38 years, 93% males) were included. Most were AIDS individuals (72.4%) under HAART (89.7%). Twenty-two (75.9%) participants had more than one HPV-OL (four with florid presentations), mostly multifocal epithelial hyperplasia (62%), being HPV-13 (26%) and HPV-32 (31%) the most frequent types. HPV load was higher in individuals with multiple HPV-OLs than in solitary lesions (4.9 vs. 3.2 Log copies/ml, p = .090) and in HPV-32 than in HPV-13 (8.3 vs. 6.4 Log copies/ml, p = .014).
Multiple HPV-OLs showed high HPV loads, possibly indicating transcriptional activity of the virus; however, in the HIV setting, the individual and local immunological response could be the key process.
尽管 HPV 已成为头颈部癌症中重要的致癌和预后生物标志物，且考虑到 HIV 个体中 HPV 相关口腔病变（HPV-OL）的增加，HPV-OL 的分子信息仍然匮乏；因此，我们的目的是确定 HIV/AIDS 个体中 HPV-OL 的病毒载量。
本横断面研究纳入了 HPV-OL 的 HIV/AIDS 患者。在获得知情同意后，进行了活检。采用 PCR 和测序（MY09/11、GP5+/6+）检测和分型 HPV。采用高分辨率熔解曲线分析（high-resolution melting assay）测定 HPV-13 和 HPV-32 的载量。采用 SPSS 软件（v.23）进行 X 2 、Fisher 确切检验和 Mann-Whitney U 检验进行统计分析。
共纳入 29 例 HIV 患者（中位年龄 38 岁，93%为男性）。大多数（72.4%）为 AIDS 个体，正在接受高效抗逆转录病毒治疗（HAART）（89.7%）。22 例（75.9%）患者有多个 HPV-OL（4 例表现为弥漫性病变），主要为多灶性上皮增生（62%），最常见的类型为 HPV-13（26%）和 HPV-32（31%）。与单发病变相比，多发 HPV-OL 患者的 HPV 载量更高（4.9 对 3.2 Log 拷贝/ml，p=0.090），HPV-32 对 HPV-13 也更高（8.3 对 6.4 Log 拷贝/ml，p=0.014）。
多发性 HPV-OL 显示出高的 HPV 载量，可能表明病毒的转录活性；然而，在 HIV 环境中，个体和局部免疫反应可能是关键过程。
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