Naumov G N, Zentsova O A, Domaradskiĭ I V
Mol Gen Mikrobiol Virusol. 1985 Mar(3):27-30.
Bacteriophage Mu genome has been transferred into the cells of Pseudomonas methanolica and Methylobacterium sp. SKF240, that are naturally resistant to the bacteriophage, as a fragment of a hybrid plasmid RP4::Mu cts62. Temperature induction of the bacteriophage results in host cell lysis. Plasmid RP::Mu cis62 is maintained in methylotrophic cells presenting a cointegrative structure. The genetic and electrophoretic, analyses of the DNA isolated from transconjugant cells have confirmed the conclusion. Bacteriophage Mu propagation has been shown to be restricted in methylotrophic cells.
噬菌体Mu基因组已作为杂交质粒RP4::Mu cts62的一个片段转入对该噬菌体天然具有抗性的甲醇假单胞菌和甲基杆菌属SKF240的细胞中。噬菌体的温度诱导导致宿主细胞裂解。质粒RP::Mu cis62以共整合结构存在于甲基营养型细胞中。对从转接合子细胞中分离的DNA进行的遗传和电泳分析证实了这一结论。已表明噬菌体Mu在甲基营养型细胞中的繁殖受到限制。
