Naumov G N, Molodova E A, Domaradskiĭ I V
Mol Gen Mikrobiol Virusol. 1986 Nov(11):44-8.
The homology region between the DNA of plasmid RP1ts::Tn601 and chromosome of the thermotolerant methylotrophic bacterium Methylobacterium sp. SKF240 has been constructed by transposon Tn601 translocation into the chromosome. The clones of Methylobacterium sp. SKF240 having integrated the plasmid RP1 into the chromosome have been obtained by conjugation on the basis of above mentioned genetic technique. The integration of plasmid RP1 into the chromosomal DNA of the methylotroph has been confirmed by the genetic and electrophoretic methods. Clones harbouring the integrated plasmid are able to transfer the chromosomal genes for methionine and isoleucine-valine synthesis to the recipient cells of P. aeruginosa PAO ML4262 by conjugation.
通过转座子Tn601易位到耐热甲基营养型细菌甲基杆菌属SKF240的染色体中，构建了质粒RP1ts::Tn601的DNA与该细菌染色体之间的同源区域。基于上述遗传技术，通过接合获得了已将质粒RP1整合到染色体中的甲基杆菌属SKF240的克隆。通过遗传和电泳方法证实了质粒RP1整合到甲基营养型细菌的染色体DNA中。携带整合质粒的克隆能够通过接合将用于甲硫氨酸和异亮氨酸 - 缬氨酸合成的染色体基因转移到铜绿假单胞菌PAO ML4262的受体细胞中。
